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Neonatal and Adult Human Intervertebral Disc scRNA-seq

ABSTRACT: Neonatal and Adult Human Intervertebral Disc scRNA-seq

PROVIDER: PRJNA785000 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17078561.fastq.gz	Fastqsanger.gz
	SRR17078562.fastq.gz	Fastqsanger.gz
	SRR17078563.fastq.gz	Fastqsanger.gz
	SRR17078564.fastq.gz	Fastqsanger.gz
	SRR17078565.fastq.gz	Fastqsanger.gz

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Project description:Intervertebral disc degeneration is a leading cause of chronic low back pain. Cell-based strategies that seek to treat disc degeneration by regenerating the central nucleus pulposus hold significant promise, but key challenges remain. One of these is the inability of therapeutic cells to effectively mimic the performance of native nucleus pulposus cells, which are unique amongst skeletal cell types in that they arise from the embryonic notochord. In this study we use single cell RNA sequencing to demonstrate emergent heterogeneity amongst notochord-derived nucleus pulposus cells in the postnatal mouse disc. Specifically, we established the existence of early and late stage nucleus pulposus cells, corresponding to notochordal progenitor and mature cells, respectively. Late stage cells exhibited significantly higher expression levels of extracellular matrix genes including aggrecan, and collagens II and VI, along with elevated TGF-β and PI3K-Akt signaling. Additionally, we identified Cd9 as a novel surface marker of late stage nucleus pulposus cells, and demonstrated that these cells were localized to the nucleus pulposus periphery, increased in numbers with increasing postnatal age, and co-localized with emerging glycosaminoglycan-rich extracellular matrix.

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