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MRNA-sequencing analysis of peripheral blood cells from an aggressive refractory pemphigus vulgaris patient

ABSTRACT: mRNA-sequencing analysis of peripheral blood cells from an aggressive refractory pemphigus vulgaris patient

PROVIDER: PRJNA785603 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17097770_1.fastq.gz	Fastqsanger.gz
	SRR17097770_2.fastq.gz	Fastqsanger.gz
	SRR17097771_1.fastq.gz	Fastqsanger.gz
	SRR17097771_2.fastq.gz	Fastqsanger.gz
	SRR17097772_1.fastq.gz	Fastqsanger.gz

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mRNA-sequencing analysis of peripheral blood cells from an aggressive refractory pemphigus vulgaris patient

Project description:Pemphigus vulgaris (PV) is a kind of IgG-mediated autoimmune blistering disease (AIBD). The peripheral immune system plays a vital role in the pathophysiology of pemphigus vulgaris. This study aimed to explore the changes of peripheral immune cells of the patient before and after medication administration.

2021-12-04 | GSE190024 | GEO

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Project description:Pemphigus obesinymphae and Pemphigus populicaulis raw sequence reads

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Gene expression analysis in canine pemphigus

Project description:We performed a comparative immunology case study of client-owned dogs to determine if immune and skin gene expression profiles in spontaneous canine pemphigus mirror those observed in human pemphigus

2021-09-20 | GSE171079 | GEO

Expression data from lymphocytes from skin of pemphigus patients and healthy control

Project description:Total RNA from lymphocytes derived from skin lesions of three pemphigus patients and three healthy control subjects was used for this study. Elevated mRNA expression levels of the immune cells activation and chemotaxis were observed in pemphigus lesions.

2019-07-30 | GSE135004 | GEO

Expression Profile of Relapsed/Refractory Aggressive B-cell Non Hodgkin Lymphoma Tumors

Project description:Expression Profile of Relapsed/Refractory Aggressive B-cell Non Hodgkin Lymphoma Tumors

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Microenvironmental network of clonal CXCL13+CD4+ T cells and regulatory T cells in pemphigus chronic blisters

Project description:Pemphigus, a rare autoimmune bullous disease mediated by anti-desmoglein autoantibodies, can be controlled with systemic medication like rituximab and high-dose systemic corticosteroids combined with immunosuppressants. However, some patients continue to experience chronically recurrent blisters in a specific area, requiring long-term maintenance systemic therapy. We demonstrate the presence of skin tertiary lymphoid structures (TLSs) with desmoglein-specific B cells in chronic blisters from pemphigus patients. In the skin TLSs, CD4+ T cells predominantly produced CXCL13. These clonally expanded CXCL13+CD4+ T cells exhibited features of activated Th1-like cells and downregulated genes associated with T-cell receptor-mediated signaling. Regulatory T cells (Tregs) are in direct contact with CXCL13+CD4+ memory T cells and increased CXCL13 production of CD4+ T cells through IL-2 consumption and TGF-β stimulation. Lastly, Intralesional corticosteroid injection improved chronic blisters and reduce skin TLSs in patients with pemphigus. This study concludes that skin TLSs are associated with the persistence of chronically recurrent blisters in pemphigus patients, and the microenvironmental network involving CXCL13+CD4+ T cells and Tregs within these structures plays an important role in CXCL13 production.

2023-09-10 | GSE242234 | GEO

Gene expression analysis in canine pemphigus

Project description:Gene expression analysis in canine pemphigus

| PRJNA718287 | ENA

Genome sequencing of the endosymbiotic consortium and mitochondrion of Pemphigus (Pemphigus) immunis species voucher ACOE3699

Project description:Genome sequencing of the endosymbiotic consortium and mitochondrion of Pemphigus (Pemphigus) immunis species voucher ACOE3699

| PRJEB59537 | ENA

Differential gene expression profiling in CD4+ T cells and CD19+ B cells between rituximab-treated complete remission (CR) and incomplete remission (IR) pemphigus patients

Project description:To understand the molecular and immunological mechanisms of remission in pemphigus patients. Remission was evaluated 5 years after rituximab onset. PBMC were purified from pemphigus whole blood patients. CD4+ T cells and CD19+ B cells were isolated by flow cytometry. Genes differentially regulated between CR and IR both in CD4+ and CD19+ cells were identified by transcriptomic approach (whole human genome).

2018-11-30 | GSE90152 | GEO

Identification of Autoreactive B Cell Subpopulations in Peripheral Blood of Autoimmune Patients With Pemphigus Vulgaris.

Project description:Pemphigus vulgaris (PV) is a rare blistering disease caused by IgG autoantibodies against the epidermal adhesion molecules desmoglein (Dsg)3 and Dsg1 providing a well-characterized paradigm of an antibody-mediated organ-specific autoimmune disease. In PV patients who have achieved clinical remission after B cell-depleting therapy, relapses often coincide with a reoccurrence of B cells and Dsg-specific autoantibodies. Here, we analyzed Dsg3-specific B cell subpopulations (i.e., total CD19+ B cells, CD19+CD27-B cells, CD19+CD27+ memory B cells, and CD19+CD27hiCD38hi plasmablasts) in peripheral blood of both PV patients (n = 14) at different stages of disease and healthy individuals (n = 14) by flow cytometry using fluorescently labeled recombinant human Dsg3 protein. Applying this approach, Dsg3-specific B cells could be detected at low frequencies (0.11-0.53% of CD19+ B cells) and numbers of Dsg3-specific memory B cells were significantly increased in PV patients in clinical remission receiving minimal immunosuppressive therapy. Finally, we confirmed in vitro that Dsg3-reactive memory B cells were able to produce anti-Dsg3 IgG autoantibodies upon ex vivo activation. Thus, monitoring of Dsg3-specific B cells in PV is of particular interest to further characterize the immunopathogenesis of PV.

| S-EPMC6587433 | biostudies-literature

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