Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

K. pneumoniae induced metabolic stress promotes immunotolerance [single-cel RNA-seq]

ABSTRACT: K. pneumoniae induced metabolic stress promotes immunotolerance [single-cel RNA-seq]

PROVIDER: PRJNA786553 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR17141911_1.fastq.gz	Fastqsanger.gz
	SRR17141911_2.fastq.gz	Fastqsanger.gz
	SRR17141912_1.fastq.gz	Fastqsanger.gz
	SRR17141912_2.fastq.gz	Fastqsanger.gz
	SRR17141913_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 16

Similar Datasets

K. pneumoniae induced metabolic stress promotes immunotolerance

Project description:K. pneumoniae induced metabolic stress promotes immunotolerance

| PRJNA765043 | ENA

K. pneumoniae induced metabolic stress promotes immunotolerance [single-cel RNA-seq]

Project description:Metabolites generated in response to infection have a major role in shaping the nature of the immune response. We predicted that the metabolic response to the highly prevalent Klebsiella pneumoniae sequence type 258 (Kp ST258) strains is responsible for their ability to persist in the lungs, despite the expected proinflammatory signaling evoked by LPS. By combining in situ metabolic imaging and comprehensive transcriptional analyses, we demonstrate that Kp ST258 activates host glutaminolysis and fatty acid oxidation (FAO), generating an oxidant-rich microenvironment predisposed to the accumulation of anti-inflammatory monocyte populations. In a setting not unlike that generated by some tumors, metabolically active Kp ST258 elicits an immunotolerant, instead of a proinflammatory response. The bacteria in turn adapt to airway oxidants by upregulation of the Type VI Secretion System (T6SS), which is highly conserved across these strains worldwide. The global success of Kp ST258 can be explained, to a major extent, by their metabolic activity that promotes a permissive immune response to which the bacteria adapt.

2022-08-09 | GSE190262 | GEO

K. pneumoniae induced metabolic stress promotes immunotolerance

2022-08-09 | GSE184563 | GEO

Klebsiella pneumoniae-induced metabolic stress promotes immunotolerance

Project description:The metabolic stress associated with K. pneumoniae ST258 infection promotes immunosuppression and drives bacterial adaptation.

| PRJEB47143 | ENA

Shear stress promotes metabolic changes in endothelial cells

Project description:Shear stress promotes metabolic changes in endothelial cells

| PRJNA835080 | ENA

CEL-Seq2: sensitive highly-multiplexed single-cell RNA-Seq

Project description:Single-cell transcriptomics requires a method that is sensitive, accurate, and reproducible. Here, we present CEL-Seq2, a modified version of our CEL-Seq method, with three-fold higher sensitivity, lower costs, and less hands-on time. We also implemented CEL-Seq2 on Fluidigm’s C1 system, thereby providing its first single-cell, on-chip barcoding method, and detected gene expression changes accompanying the progression through the cell cycle in mouse fibroblast cells. We also compare with Smart-Seq to demonstrate CEL-Seq2’s increased sensitivity relative to other available methods. Collectively, the improvements make CEL-Seq2 uniquely suited to single-cell RNA-Seq analysis in terms of economics, resolution, and ease of use

2016-04-08 | GSE78779 | GEO

Bap1 promotes osteoclast function by metabolic reprogramming

Project description:Bap1 promotes osteoclast function by metabolic reprogramming

| PRJNA967738 | ENA

Dicyemida sp. CEL-2019

Project description:Dicyemida sp. CEL-2019 Raw sequence reads

| PRJNA531422 | ENA

Shear stress promotes metabolic changes in endothelial cells

Project description:Here we investigated how shear stress influences the metabolic behavior of endothelial cells (ECs) using a combination of transcriptomics, tracer metabolomics. Transcriptomics analysis of ECs under wall shear stress (WSS) showed an increase in the glutamine, asparagine, alanine, aspartate, and glutamate biochemical activity while the downregulated gene signature suggested a reduction of the glycolysis activity. Metabolomics analysis of the medium from static and WSS cultured ECs revealed that -upon WSS- changes in the nutritional preferences of ECs occurred. Using tracer metabolomics, we further evidenced that WSS promotes glutamine anaplerosis into the Krebs cycle and a decrease in the glycolytic metabolism of ECs in vitro. In conclusion, our findings elucidate the nutritional preferences of ECs and reveal the importance of a physical stimulus (WSS) in the regulation of EC metabolism in vitro.

2023-12-01 | GSE202220 | GEO

Solenopharyngidae sp. CEL-2015

Project description:Solenopharyngidae sp. CEL-2015 Transcriptome or Gene expression

| PRJNA277592 | ENA