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Bacterial growth in multicellular aggregates leads to the emergence of complex lifecycles

ABSTRACT: Bacterial growth in multicellular aggregates leads to the emergence of complex lifecycles

PROVIDER: PRJNA786643 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17149641_1.fastq.gz	Fastqsanger.gz
	SRR17149641_2.fastq.gz	Fastqsanger.gz
	SRR17149642_1.fastq.gz	Fastqsanger.gz
	SRR17149642_2.fastq.gz	Fastqsanger.gz
	SRR17149643_1.fastq.gz	Fastqsanger.gz

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Bacterial growth in multicellular aggregates leads to the emergence of complex lifecycles

Project description:In response to environmental stresses such as starvation, many bacteria facultatively aggregate into multicellular structures that can attain new metabolic functions and behaviors. Despite the ubiquity and relevance of this form of collective behavior, we lack an understanding of how the spatiotemporal dynamics of aggregate development emerge from cellular physiology. Here, we study the development of multicellular aggregates by the marine bacterium Vibrio splendidus when it grows on the polysaccharide alginate. Transcriptional profiling was used to define genes differentially expressed at stages of aggregate morphogenesis, and between cell sub-populations, which included genes encoding a putative type IV pillus and carbon storage granules. Combined with measurements of in situ cellular physiology, we show that the coupling between growth and spatial gradient formation leads to the emergence of a complex lifecycle.

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Bacterial growth in multicellular aggregates leads to the emergence of complex life cycles.

Project description:Facultative multicellular behaviors expand the metabolic capacity and physiological resilience of bacteria. Despite their ubiquity in nature, we lack an understanding of how these behaviors emerge from cellular-scale phenomena. Here, we show how the coupling between growth and resource gradient formation leads to the emergence of multicellular lifecycles in a marine bacterium. Under otherwise carbon-limited growth conditions, Vibrio splendidus 12B01 forms clonal multicellular groups to collectively harvest carbon from soluble polymers of the brown-algal polysaccharide alginate. As they grow, groups phenotypically differentiate into two spatially distinct sub-populations: a static "shell" surrounding a motile, carbon-storing "core." Differentiation of these two sub-populations coincides with the formation of a gradient in nitrogen-source availability within clusters. Additionally, we find that populations of cells containing a high proportion of carbon-storing individuals propagate and form new clusters more readily on alginate than do populations with few carbon-storing cells. Together, these results suggest that local metabolic activity and differential partitioning of resources leads to the emergence of reproductive cycles in a facultatively multicellular bacterium.

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