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Discovery of neuro- and vascular-associated periosteum-resident macrophages essential for cortical bone regeneration [neg vs pos]

ABSTRACT: Discovery of neuro- and vascular-associated periosteum-resident macrophages essential for cortical bone regeneration [neg vs pos]

PROVIDER: PRJNA787255 | ENA |

REPOSITORIES: ENA

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Discovery of neuro- and vascular-associated periosteum-resident macrophages essential for cortical bone regeneration [neg vs pos]

Project description:The periosteum contains a highly osteogenic and neuro-vascular microenvironment that is essential for cortical bone formation and regeneration. Resident tissue macrophages (RTMs) are critical for maintaining tissue-specific niches and participating in tissue regeneration. However, the characteristics, origin and functions of periosteum-resident macrophages (PRMs) remain largely unknown. In this study, we demonstrated that PRMs are generated during embryonic hematopoiesis and are self-maintained locally during regeneration. Furthermore, by single-cell RNA sequencing analysis of periosteum myeloid cells, CX3CR1+CD45+ACE+ and CX3CR1+CD45+CD74+ cells were identified as vascular-associated and neuro-associated PRMs, respectively. Both cell types were found to arise from CX3CR1+CD168+CD45+ PRMs and shown to play critical roles in maintaining the periosteum neuro-vascular niche and promoting early-stage cortical bone regeneration. Importantly, the neuro-vascular characteristic of PRMs are directly modulated via Periostin, the essential ECM distributed in periosteum. These findings elucidate the characteristics and origins of PRMs and reveal their essential roles in maintaining the local niche and cortical bone regeneration as well as highlighting the potential value of PRMs as a therapeutic target in bone regeneration.

2024-06-01 | GSE190476 | GEO

Discovery of neuro- and vascular-associated periosteum-resident macrophages essential for cortical bone regeneration scRNA

2024-06-01 | GSE190483 | GEO

Discovery of neuro- and vascular-associated periosteum-resident macrophages essential for cortical bone regeneration [veh vs pos]

2024-06-01 | GSE190478 | GEO

Discovery of neuro- and vascular-associated periosteum-resident macrophages essential for cortical bone regeneration [veh vs pos]

Project description:Discovery of neuro- and vascular-associated periosteum-resident macrophages essential for cortical bone regeneration [veh vs pos]

| PRJNA787260 | ENA

Discovery of neuro- and vascular-associated periosteum-resident macrophages essential for cortical bone regeneration

Project description:Discovery of neuro- and vascular-associated periosteum-resident macrophages essential for cortical bone regeneration

| PRJNA787270 | ENA

Discovery of neuro- and vascular-associated periosteum-resident macrophages essential for cortical bone regeneration scRNA

Project description:Discovery of neuro- and vascular-associated periosteum-resident macrophages essential for cortical bone regeneration scRNA

| PRJNA787272 | ENA

Discovery of neuro- and vascular-associated periosteum-resident macrophages essential for cortical bone regeneration

Project description:This SuperSeries is composed of the SubSeries listed below.

2024-06-01 | GSE190485 | GEO

Comparative transcriptomics reveals circadian and pluripotency networks as two pillars of longevity regulation

Project description:Mammals differ more than 100-fold in maximum lifespan. Here, we conducted comparative transcriptomics on 26 species with diverse lifespans. We identified thousands of genes with expression levels negatively or positively correlated with a species' maximum lifespan (Neg- or Pos-MLS genes). Neg-MLS genes are primarily involved in energy metabolism and inflammation. Pos-MLS genes show enrichment in DNA repair, microtubule organization, and RNA transport. Expression of Neg- and Pos-MLS genes is modulated by interventions, including mTOR and PI3K inhibition. Regulatory networks analysis showed that Neg-MLS genes are under circadian regulation possibly to avoid persistent high expression, whereas Pos-MLS genes are targets of master pluripotency regulators OCT4 and NANOG and are upregulated during somatic cell reprogramming. Pos-MLS genes are highly expressed during embryogenesis but significantly downregulated after birth. This work provides targets for anti-aging interventions by defining pathways correlating with longevity across mammals and uncovering circadian and pluripotency networks as central regulators of longevity.

2022-05-18 | GSE190756 | GEO

Effect of loss of Gfi1b on hematopietic stem cells

Project description:Using Gfi1b conditional mice, deletion of gfi1b in the hematopietic system was induced by injecting MxCre tg Gfi1bfl/fl mice with pIpC. 30 days after injection, Cd150 pos, Cd 48 neg, Lin neg Sca and c-kit pos stem cells were sortrted from Gfi1bfl/fl and Mxcre tg Gfi1bfl/fl mice and analysed. We used the mouse Affymetrix Gene ST Array.

2010-09-03 | GSE20655 | GEO

Periosteum contains skeletal stem cells with high bone regenerative potential controlled by Periostin

Project description:Bone regeneration relies on the activation of skeletal stem cells (SSCs) that still remain poorly characterized. Here, we show that periosteum contains SSCs with high bone regenerative potential compared to bone marrow stromal cells/skeletal stem cells (BMSCs) in mice. Although periosteal cells (PCs) and BMSCs are derived from a common embryonic mesenchymal lineage, post-natally PCs exhibit greater clonogenicity, growth and differentiation capacity than BMSCs. During bone repair, PCs can efficiently contribute to cartilage and bone, and integrate long-term after transplantation. Molecular profiling uncovers genes encoding Periostin and other extracellular matrix molecules associated with the enhanced response to injury of PCs. Periostin gene deletion impairs PC functions and fracture consolidation. Periostin-deficient periosteum cannot reconstitute a pool of PCs after injury demonstrating the presence of SSCs within periosteum and the requirement of Periostin in maintaining this pool. Overall our results highlight the importance of analyzing periosteum and PCs to understand bone phenotypes.

2018-10-18 | E-MTAB-6417 | biostudies-arrayexpress

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