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Discovery of neuro- and vascular-associated periosteum-resident macrophages essential for cortical bone regeneration [veh vs pos]

ABSTRACT: Discovery of neuro- and vascular-associated periosteum-resident macrophages essential for cortical bone regeneration [veh vs pos]

PROVIDER: PRJNA787260 | ENA |

REPOSITORIES: ENA

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Discovery of neuro- and vascular-associated periosteum-resident macrophages essential for cortical bone regeneration [veh vs pos]

Project description:The periosteum contains a highly osteogenic and neuro-vascular microenvironment that is essential for cortical bone formation and regeneration. Resident tissue macrophages (RTMs) are critical for maintaining tissue-specific niches and participating in tissue regeneration. However, the characteristics, origin and functions of periosteum-resident macrophages (PRMs) remain largely unknown. In this study, we demonstrated that PRMs are generated during embryonic hematopoiesis and are self-maintained locally during regeneration. Furthermore, by single-cell RNA sequencing analysis of periosteum myeloid cells, CX3CR1+CD45+ACE+ and CX3CR1+CD45+CD74+ cells were identified as vascular-associated and neuro-associated PRMs, respectively. Both cell types were found to arise from CX3CR1+CD168+CD45+ PRMs and shown to play critical roles in maintaining the periosteum neuro-vascular niche and promoting early-stage cortical bone regeneration. Importantly, the neuro-vascular characteristic of PRMs are directly modulated via Periostin, the essential ECM distributed in periosteum. These findings elucidate the characteristics and origins of PRMs and reveal their essential roles in maintaining the local niche and cortical bone regeneration as well as highlighting the potential value of PRMs as a therapeutic target in bone regeneration.

2024-06-01 | GSE190478 | GEO

Discovery of neuro- and vascular-associated periosteum-resident macrophages essential for cortical bone regeneration scRNA

2024-06-01 | GSE190483 | GEO

Discovery of neuro- and vascular-associated periosteum-resident macrophages essential for cortical bone regeneration [neg vs pos]

2024-06-01 | GSE190476 | GEO

Discovery of neuro- and vascular-associated periosteum-resident macrophages essential for cortical bone regeneration [neg vs pos]

Project description:Discovery of neuro- and vascular-associated periosteum-resident macrophages essential for cortical bone regeneration [neg vs pos]

| PRJNA787255 | ENA

Discovery of neuro- and vascular-associated periosteum-resident macrophages essential for cortical bone regeneration

Project description:Discovery of neuro- and vascular-associated periosteum-resident macrophages essential for cortical bone regeneration

| PRJNA787270 | ENA

Discovery of neuro- and vascular-associated periosteum-resident macrophages essential for cortical bone regeneration scRNA

Project description:Discovery of neuro- and vascular-associated periosteum-resident macrophages essential for cortical bone regeneration scRNA

| PRJNA787272 | ENA

Discovery of neuro- and vascular-associated periosteum-resident macrophages essential for cortical bone regeneration

Project description:This SuperSeries is composed of the SubSeries listed below.

2024-06-01 | GSE190485 | GEO

Periosteum contains skeletal stem cells with high bone regenerative potential controlled by Periostin

Project description:Bone regeneration relies on the activation of skeletal stem cells (SSCs) that still remain poorly characterized. Here, we show that periosteum contains SSCs with high bone regenerative potential compared to bone marrow stromal cells/skeletal stem cells (BMSCs) in mice. Although periosteal cells (PCs) and BMSCs are derived from a common embryonic mesenchymal lineage, post-natally PCs exhibit greater clonogenicity, growth and differentiation capacity than BMSCs. During bone repair, PCs can efficiently contribute to cartilage and bone, and integrate long-term after transplantation. Molecular profiling uncovers genes encoding Periostin and other extracellular matrix molecules associated with the enhanced response to injury of PCs. Periostin gene deletion impairs PC functions and fracture consolidation. Periostin-deficient periosteum cannot reconstitute a pool of PCs after injury demonstrating the presence of SSCs within periosteum and the requirement of Periostin in maintaining this pool. Overall our results highlight the importance of analyzing periosteum and PCs to understand bone phenotypes.

2018-10-18 | E-MTAB-6417 | biostudies-arrayexpress

Single nuclei RNAseq of the human periosteum from patients with congenital pseudarthrosis of the tibia

Project description:Congenital pseudarthrosis of the tibia (CPT) is a severe pediatric disorder affecting children. CPT is characterized by tibial bowing at birth leading to spontaneous fracture and fibrous non-union. Our study showed the presence of biallelic inactivation of the NF1 gene in the periosteum at the pseudarthrosis site. To understand the impact of NF1 mutation on the periosteum, we performed single nuclei RNAseq of pathological periosteum collected from the pseudarthrosis site and of non-pathological periosteum collected from the iliac crest of 3 CPT patients.

2024-07-24 | GSE232516 | GEO

Gene expression in alpha SMA Cre-labeled cells in periosteum during early fracture callus formation

Project description:Fracture healing is a process that involves many cell populations. In this study we characterized gene expression in a subset of cells involved in fracture healing. αSMACreERT2 mice crossed with Ai9 reporter mice that express tdTomato fluorescent protein after Cre-mediated activation were used as an experimental model. αSMA-expressing cells were labeled by tamoxifen administration, then periosteal cells from the tibia were isolated two days later (controls), or tibial fractures were performed and periosteum/soft callus tissue was collected after 2 and 6 days. The tdTomato positive cell population was isolated by flow cytometry, and subjected to microarray analysis. Histology and cell surface marker analysis indicates that αSMACreERT2 labels a mainly mesenchymal population in the periosteum that expands after fracture, and contributes to both osteogenic and chondrogenic elements of the fracture callus. We were therefore able to examine gene expression in a defined population during the early stages of fracture healing. Total RNA was obtained from the tomato positive cells within the periosteal compartment of fractures from αSMACreERT2/Ai9 mice. Control animals were given 2 doses of tamoxifen, and periosteum was collected and labeled cells sorted (8-9 sex-matched mice per group). Fractures were performed after the second dose of tamoxifen, and tomato positive cells from periosteum/callus tissue were isolated 2 and 6 days after fracture (4-8 animals per sample pooled). 3 replicates for each sample are included.

2013-11-27 | E-GEOD-45156 | biostudies-arrayexpress

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