Ontology highlight
ABSTRACT:
PROVIDER: PRJNA787660 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Strigolactones (SLs) are plant hormones that regulate diverse developmental processes and environmental responses in plants. It has been discovered that SLs play an important role in regulating plant immune resistance to pathogens, but there are currently no reports on their role in the interaction between Nicotiana benthamiana and Tobacco mosaic virus (TMV). In this study, the exogenous application of SLs weakened the resistance of N. benthamiana to TMV, promoting TMV infection, whereas the exogenous application of Tis108, an SL inhibitor, resulted in the opposite effect. Virus-induced gene silencing (VIGS) inhibition of two key SL synthesis enzyme genes, NtCCD7 and NtCCD8, enhanced the resistance of N. benthamiana to TMV. Additionally, we conducted a screening of N. benthamiana related to TMV infection. TMV-infected plants treated with SLs were compared to the control by using RNA-seq. KEGG enrichment analysis and weighted gene co-expression network analysis (WGCNA) of differentially expressed genes (DEGs) suggested that plant hormone signaling transduction may play a significant role in the SL-TMV-N. benthamiana interactions. This study reveals new functions of SLs in regulating plant immunity and provides a reference for controlling TMV diseases in production.
2024-08-16 | GSE272661 | GEO
Project description:Compare gene expression between maize genotype resistant (Pa405) and susceptible (Oh28) to maize dwarf mosaic virus (MDMV) infection 4 days post-inoculation using microarrays.
2013-07-07 | E-MTAB-1485 | biostudies-arrayexpress
Project description:To understand molecular pathogenesis of chemical induced contact dermatitis, cultured normal human epidermal keratinocytes (NHKCs) were treated with sub-cytotoxic concentration of sodium lauryl sulfate (SLS, 10 microM) and urushiol (1 microM). In analysis, 259 genes were selected as up-regulated DEGs in the SLS-treated NHKCs and 193 genes as down-regulated DEGs. In urushiol-treated NHKCs, 173 up-regulated and 124 down-regulated DEGs were identified. Gene Ontology (GO) biological process (BP) in the SLS-induced upregulated DEGS in NHKCs were inflammation-associated biological processes, such as inflammatory response (GO:0006954), cellular response to calcium ion (GO:0071277), immune response (GO:0006955) and cellular response to lipopolysaccharide (GO:0071222) and in urushiol-induced upregulated DEGs were inflammation-associated biological processes such as cytokine-mediated signaling pathway (GO:0019221) and inflammatory response (GO:0006954). SLS downregulated proteins in chemotaxis (GO:0006935), oxidation-reduction process (GO:0055114), cornification (GO:0070268), G1/S transition of mitotic cell cycle (GO:0000082), keratinization (GO:0031424) and cellular protein metabolic process (GO:0044267) and urushiol downregulated proteins in nervous system development (GO:0007399), positive regulation of cell proliferation (GO:0008284) and cellular protein metabolic process (GO:0044267) significantly.
2019-11-01 | GSE126354 | GEO
Project description:Seed germination is a critical developmental process in plant propagation. Knowledge of the gene expression patterns in this critical process is important in order to understand the main biochemical reactions involved in successful germination, specially for economically relevant plants such as Maize. The purpose of this study is the gene expression analysis of quiescent and germinated maize embryonic axes to determine the regulatory mechanism for (r)-protein expression during the maize development process.
2011-08-01 | GSE27648 | GEO
Project description:Seed germination is a critical developmental process in plant propagation. Knowledge of the gene expression patterns in this critical process is important in order to understand the main biochemical reactions involved in successful germination, specially for economically relevant plants such as Maize. The purpose of this study is the gene expression analysis of quiescent and germinated maize embryonic axes to determine the regulatory mechanism for (r)-protein expression during the maize development process. This study analyzed samples of total and polysomal RNA from maize embryonic axes at different time periods during the germination process and under the influence of a specific growth factor. For each condition a biological replicate was included and for the time periods, a technical replicate was also included.
2011-07-31 | E-GEOD-27648 | biostudies-arrayexpress
Project description:Ustilago maydis is a basidiomycete fungus that causes smut disease in maize. Most prominent symptoms of the disease are plant tumors, which can be induced by U. maydis on all aerial parts of the plant. We identified two linked genes, pit1 and pit2, which are specifically expressed during plant colonization. Deletion mutants for either pit1 or pit2 are unable to induce tumor development and elicit plant defense responses. We used the Affymetrix maize genome array to analyze the transcriptional responses of maize to deletion pit1 and pit2 mutants and found plant responses to both mutants being not significantly distinguishable.
2011-07-01 | GSE28479 | GEO
Project description:Using high-throughput RNA sequencing, we developed a spatiotemporal transcriptome atlas for seed development of eight maize inbred lines based on 144 samples from the middle to late stages of grain development. A total of 26,747 genes with FPKM value more than 1 at least one sample were found to be involved in programming grain development. Global comparisons of genes expression highlighted the fundamental transcriptomic reprogramming and the phases of development. Coexpression analysis provided further insight into the dynamic reprogramming of the transcriptome by revealing functional transitions during maturation. Combined with grain moisture content and grain dehydration rate of different developmental time points of eight maize inbred lines, we captured a large number of genes related to grain moisture content and grain dehydration rate, which should help elucidate key mechanisms and regulatory networks that underlie grain dehydration during maize grain development. These results provide a comprehensive understanding of which biological processes are involved in the regulation of moisture variety of maize grain, the general principles of which provide a new perspective on improving maize grain dehydration characteristics. Meanwhile, this study provides a valuable resource for understanding the genetic regulation of maize grain development.
2022-10-01 | GSE158816 | GEO