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SMARCA4-dependent regulation of chromatin and transcription in neuroblastoma

ABSTRACT: SMARCA4-dependent regulation of chromatin and transcription in neuroblastoma

PROVIDER: PRJNA788135 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17205229_1.fastq.gz	Fastqsanger.gz
	SRR17205229_2.fastq.gz	Fastqsanger.gz
	SRR17205230_1.fastq.gz	Fastqsanger.gz
	SRR17205230_2.fastq.gz	Fastqsanger.gz
	SRR17205231_1.fastq.gz	Fastqsanger.gz

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Project description:To reveal the genome-wide targets of SWI/SNF complexes in neuroblastoma cells, we performed ATAC-seq in IMR-32 cells with or without SMARCA4 inactivation. To identify changes in DNA accessibility following SMARCA4 inactivation, we used either the small-molecule catalytic inhibitor BRM014, auxin-induced degradation of IMR-32 cells engineered with SMARCA4 tagged to the minimal auxin-induced degron (SMARCA4-mAID), or the corresponding vehicle controls. Analysis of these locations reveal that SMARCA4-dependent sites are located at enhancers used by the neuroblastoma core regulatory circuitry.

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Genome-wide profiling of SMARCA4 in neuroblastoma cells [ChIP-seq]

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