Micro-RNA expression profile of BALB/c mouse glandular stomach in the early phase of Cryptosporidium muris infection
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PROVIDER: PRJNA788286 | ENA |
REPOSITORIES: ENA
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Project description:Cryptosporidiosis is a zoonotic disease caused by infection with the oocyst of Cryptosporidium in human and animals. MicroRNA (miRNA) emerges as important player in regulating the innate immune response against parasitic infection. Here, we compared miRNA profiles of the glandular stomach of Cryptosporidium muris (C. muris) infected and un-infected BALB/c mice using microarray sequencing. A total of 10 miRNAs (including 3 upregulated and 7 downregulated miRNAs) with significant expression differences (|FC| ≥ 2 and P value test < 0.05) were screened after C. muris infected the glandular stomach of BALB/c mice for 8 hours. MiRWalk and miRDB online bioinformatics software were used to predict the target genes of differentially expressed miRNAs. Gene Ontology (GO) and KEGG enrichment analyses were performed for annotate the target genes. GO terms indicates that many are associated with the relevant generic transcription and ion transport. In addition, the KEGG analyses showed that the target genes were strictly related to a diverse types of tumor disease progression and the antipathogen immunity pathway. In the current study, we first reported the changes of miRNA expression profile in glandular stomach of BALB/c mice at the early phase of C. muris invasion. As such, dysregulation of miRNA expression profile may contribute to our understanding of the Cryptosporidiosis pathology. The result reported in this paper provide a new perspective into the miRNA regulatory mechanisms of Cryptosporidiosis, which may help to develop effective control strategies against Cryptosporidium.
2022-12-31 | GSE190734 | GEO
Project description:A pathogen of rodents predominantly, this organism is found in the gastric glands of the host's stomach.
| PRJNA28945 | ENA
Project description:Gan mice express Wnt1, Ptgs2, and Ptges, which develop inflammation-associated gastric tumors (Oshima et al, Gastroenterology 131: 1086, 2006). We examined the role of TNF-alpha in tumorigenesis by construction of TNF-/- Gan mice. We also examined genetic background difference in tumor phenotype by changing Gan mouse background from C57BL/6(B6) to BALB/c. Microarray analyses were performed to examine changes of expression profiles in tumors by TNF gene disruption or by changing genetic background. Total RNA was prepared from B6 Gan mice (n=3: Gan1-Gan3), B6-Gan TNF-/- mice (n=3: Gan(TNF KO)1-Gan(TNF KO)3), BALB-Gan mice (n=3: Gan(BALB/c)1-Gan(BALB/c)3), and wid-type normal glandular stomach (n=3: WT1-WT3). We used Affymetrix microarrays for hybridization, and examined expression profiles.
2012-12-27 | E-GEOD-43145 | biostudies-arrayexpress
Project description:GATA4 facilitates its function as a transcriptional regulator by binding to promoter and enhancer regions of target genes in the glandular stomach.
2020-08-13 | GSE156131 | GEO
Project description:Trichuris muris is very closely related to the human parasite T. trichiura sharing cross reactive antigens. Moreover, it is a remarkably tractable model system for dissecting immune responses and host parasite relationships and is actively being investigated in a number of laboratories worldwide. T. muris is a naturally occurring nematode parasite of mice which resides in the caecum and colon and has a direct oral faecal life cycle. High-throughput sequencing of Trichuris muris transcriptome for de novo assembly of transcripts. The main objective of this project is to recognize genes expressed in given life stages. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
2013-05-28 | E-ERAD-125 | biostudies-arrayexpress
Project description:Cryptosporidium is a leading cause of diarrheal disease and mortality in young children worldwide. Cryptosporidium invades small intestinal epithelial cells and forms a unique intracellular niche, a process that may alter gut microbes and their production metabolites. However, the mechanism of interactions between gut microbes, metabolites, and parasites is poorly understood. Here, we first characterized the impacts of Cryptosporidium infection on gut microbiota using a microbiome-to-metabolome association study. BALB/c mice were gavaged with Cryptosporidium muris, and fecal samples were collected at 0, 7, 14, 21, and 28 days postinfection (dpi) to observe changes in the intestinal microbes of the body during parasite infection. The infected group had a significantly increased relative abundance of bacterial taxa, such as Lachnospiraceae and Prevotella (P < 0.05), associated with the biosynthesis of short-chain fatty acids (SCFAs). Metabolites related to the metabolic pathways, steroid hormone biosynthesis, and biosynthesis of unsaturated fatty acids pathway were upregulated at 7 dpi, indicating that related metabolites in the biosynthesis of unsaturated fatty acids may be essential for C. muris reproduction in vivo. The metabolites involved in metabolic pathways, bile secretion, and primary bile acid biosynthesis were upregulated at 14 dpi, and we speculate that these metabolites may be critical to the growth and development of Cryptosporidium oocysts in the host. Correlation analysis revealed that Firmicutes bacteria are significantly associated with α-linolenic acid metabolism pathways (P< 0.05). The gut microbiota changes dynamically, and the metabolites involved in fatty acid and bile acid biosynthesis may play important roles during cryptosporidiosis. Details of the gut microbiota and the metabolome after infection with Cryptosporidium may aid in the discovery of specific diagnostic markers and help us understand the changes in parasite metabolic pathways. IMPORTANCE Cryptosporidiosis is a gastrointestinal disease in humans and animals caused by the protozoan parasite Cryptosporidium. Control and treatment of the disease is challenging due to the lack of sensitive diagnostic tools and effective chemotherapy. The dynamics of gut microbiota and metabolites during Cryptosporidium infection may be the key to finding drugs and targets for parasite infection control. Our results indicate that C. muris infection can disrupt gut microbiota and metabolites, resulting in decreased bacterial abundance at the parasitic site. Unsaturated fatty acid pathway biosynthesis-related metabolites are significantly elevated at the patent period. Interestingly, the metabolite pathway that significantly elevated during peak parasite growth was bile acid, the metabolites of which may be important for the circulation of infection of Cryptosporidium oocysts in the host. The enhancing effects of short-chain fatty acid and bile acid metabolism on the growth and development of Cryptosporidium proposed in this study may provide a theoretical basis for future research on novel drugs and vaccines against this intestinal parasite.
| S-EPMC9927150 | biostudies-literature
Project description:Tabula Muris Senis is a single cell transcriptomic atlas of 18 tissues and organs from Mus musculus across the organism’s life span.
2020-04-30 | GSE149590 | GEO
Project description:The in vivo function of AGR2 was examined by generating an AGR2 null mouse. The mice died prematurely due to hyperplasia and dysplasia of the glandular stomach. Gene expression profiling was performed to compare differences in transcription between wild-type and null AGR2 mice. Female AGR2 wild-type and knockout mice were sacrificed at the age of 26 weeks and the glandular stomachs were removed. Total RNA was isolated using TRIzol reagent (Invitrogen, Carlsbad, CA). The RNA was was reverse transcribed, labeled, and hybridized to mouse oligonucleotide DNA microarrays (Illumina MouseRef-8 v2 Expression BeadChip)
2012-12-01 | E-GEOD-40062 | biostudies-arrayexpress