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P. syringae pv tomato DC3000 AlgU in planta regulon RNA-seq data

ABSTRACT: P. syringae pv tomato DC3000 AlgU in planta regulon RNA-seq data

PROVIDER: PRJNA789487 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17247300.fastq.gz	Fastqsanger.gz
	SRR17247301.fastq.gz	Fastqsanger.gz
	SRR17247302.fastq.gz	Fastqsanger.gz
	SRR17247303.fastq.gz	Fastqsanger.gz
	SRR17247304.fastq.gz	Fastqsanger.gz

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P. syringae pv tomato DC3000 AlgU in planta regulon RNA-seq data

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Project description:Plant pathogenic bacteria encounter a drastic increase in apoplastic pH during the early stages of plant immunity. The effects of alkalization on pathogen-host interactions have not been comprehensively characterized. Here we used a global transcriptomic approach to assess the impact of environmental alkalization on Pseudomonas syringae pv. tomato DC3000 in vitro. In addition to the Type 3 Secretion System (T3SS), we found expression of genes encoding other virulence factors such as iron uptake, and coronatine biosynthesis to be strongly affected by environmental alkalization. We also found activity of extracytoplasmic function sigma factor, AlgU, was induced at pH 5.5 and suppressed at pH 7.8, which are pH levels that this pathogen would likely experience before and during pattern triggered immunity, respectively. This pH-dependent control requires the presence of periplasmic proteases, AlgW and MucP, that function as part of the environmental sensing system that activates AlgU in specific conditions. This is the first example of pH-dependency of AlgU activity, suggesting a regulatory pathway model where pH affects the proteolysis-dependent activation of AlgU. These results contribute to deeper understanding of the role apoplastic pH has on host-pathogen interactions.

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Project description:This experiment analyses the expression data of the wild type P. syringae pv. tomato DC3000 grown in the absence and in the presence of phloretin and naringenin.

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Project description:This experiment analyses the expresssion data of the wild type P. syringae pv. tomato DC3000 compared with its fleQ mutant grown under two different conditions: liquid culture in minimal medium and swarming plates.

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In planta transcriptome analysis of Pseudomonas syringae pv. tomato DC3000 during early infection and early exposure to pattern-triggered immunity in Arabidopsis thaliana

Project description:We develop a protocol for extracting P. syringae transcriptome from infected A. thaliana tissue through physical separation by low speed centrifugation. We use this protocol to compare the RNASeq transcriptome profiles of DC3000 during naïve host infection and exposure to pattern triggered immunity at 1, 3, and 5 hours post inoculation

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Biophysical and proteomic analyses suggest functions of Pseudomonas syringae pv tomato DC3000 extracellular vesicles in bacterial growth during plant infection

Project description:Vesiculation is a process employed by Gram-negative bacteria to release extracellular vesicles (EVs) into the environment. Bacterial EVs contain molecular cargo from the donor bacterium and play important roles in bacterial survival and growth. Here, we describe EV production in plant-pathogenic Pseudomonas syringae pv. tomato DC3000 (Pto DC3000), the causal agent of bacterial speck disease. Cultured Pto DC3000 exhibited EV structures both on the cell surface and in the vicinity of bacterial cells, observed as outer membrane vesicle (OMV) release. We used in-solution trypsin digestion coupled to mass spectrometry to identify 369 proteins enriched in EVs recovered from cultured Pto DC3000. The predicted localization profile of EV proteins supports the production of EVs also in the form of outer-inner-membrane vesicles (OIMVs). EV production varied slightly between bacterial lifestyles and also occurred in planta. The potential contribution of EVs to Pto DC3000 plant infection was assessed using plant treatments and bioinformatic analysis of the EV-enriched proteins. While these results identify immunogenic activities of the EVs, they also point at roles for EVs in bacterial defences and nutrient acquisition by Pto DC3000.

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