Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptional Profiling of Early and Late Phases of Bovine Tuberculosis

ABSTRACT: Transcriptional Profiling of Early and Late Phases of Bovine Tuberculosis

PROVIDER: PRJNA791899 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR17319913.fastq.gz	Fastqsanger.gz
	SRR17319914.fastq.gz	Fastqsanger.gz
	SRR17319915.fastq.gz	Fastqsanger.gz
	SRR17319916.fastq.gz	Fastqsanger.gz
	SRR17319917.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 48

Similar Datasets

Transcriptional Profiling of Early and Late Phases of Bovine Tuberculosis

Project description:We report RNAseq analysis of Early and Late Phases of Bovine Tuberculosis

2021-12-30 | GSE192537 | GEO

CMV-CTL RNA-seq at the early & late phases

Project description:CMV-CTL RNA-seq at the early & late phases

| PRJNA741901 | ENA

Transcriptional Profiling of Early and Late Phases of Bovine Tuberculosis.

Project description:Bovine tuberculosis, caused by Mycobacterium tuberculosis var. bovis (M. bovis), is an important enzootic disease affecting mainly cattle, worldwide. Despite the implementation of national campaigns to eliminate the disease, bovine tuberculosis remains recalcitrant to eradication in several countries. Characterizing the host response to M. bovis infection is crucial for understanding the immunopathogenesis of the disease and for developing better control strategies. To profile the host responses to M. bovis infection, we analyzed the transcriptome of whole blood cells collected from experimentally infected calves with a virulent strain of M. bovis using RNA transcriptome sequencing (RNAseq). Comparative analysis of calf transcriptomes at early (8 weeks) versus late (20 weeks) aerosol infection with M. bovis revealed a divergent and unique profile for each stage of infection. Notably, at the early time point, transcriptional upregulation was observed among several of the top-ranking canonical pathways involved in T-cell chemotaxis. At the late time point, enrichment in the cell mediated cytotoxicity (e.g., Granzyme B) was the predominant host response. These results showed significant change in bovine transcriptional profiles and identified networks of chemokine receptors and monocyte chemoattractant protein (CCL) coregulated genes that underline the host-mycobacterial interactions during progression of bovine tuberculosis in cattle. Further analysis of the transcriptomic profiles identified potential biomarker targets for early and late phases of tuberculosis in cattle. Overall, the identified profiles better characterized identified novel immunomodulatory mechanisms and provided a list of targets for further development of potential diagnostics for tuberculosis in cattle.

| S-EPMC8853682 | biostudies-literature

Pathogen Detection in Early Phases of Experimental Bovine Tuberculosis.

Project description:Bovine tuberculosis is caused by Mycobacterium bovis, a member of the M. tuberculosis complex of mycobacterial species that cause tuberculosis in humans and animals. Diagnosis of bovine tuberculosis has relied on examinations of cell-mediated immune responses to M. bovis proteins using tuberculin skin testing and/or interferon gamma release assays. Even when using these methods, disease detection during the earliest phases of infection has been difficult, allowing a window for cattle-to-cattle transmission to occur within a herd. Alternative means of diagnosis could include methods to detect M. bovis or M. bovis DNA in bodily fluids such as nasal secretions, saliva, or blood. During the first 8 weeks after experimental aerosol infection of 18 calves, M. bovis DNA was detected in nasal swabs from a small number of calves 5, 6, and 8 weeks after infection and in samples of saliva at 1, 7, and 8 weeks after infection. However, at no time could culturable M. bovis be recovered from nasal swabs or saliva. M. bovis DNA was not found in blood samples collected weekly and examined by real-time PCR. Interferon gamma release assays demonstrated successful infection of all calves, while examination of humoral responses using a commercial ELISA identified a low number of infected animals at weeks 4-8 after infection. Examination of disease severity through gross lesion scoring did not correlate with shedding in nasal secretions or saliva, and calves with positive antibody ELISA results did not have more severe disease than other calves.

| S-EPMC11359862 | biostudies-literature

Early response of bovine alveolar macrophages to infection with live and heat-killed Mycobacterium bovis

Project description:Bovine tuberculosis, caused by Mycobacterium bovis, is a disease of considerable economic importance yet comparatively little is known about the bovine immune response to the disease. Alveolar macrophages are one of the first cells to encounter mycobacteria following infection. In this experiment we investigated the early transcriptional response of bovine alveolar macrophages following infection with M. bovis. The transcriptional response to heat-killed M. bovis was also investigated to look for genes that are only differentially transcribed in response to the live organism.

2010-01-31 | GSE19237 | GEO

Single cell RNA-seq for human and bovine PBMCs stimulated with M. tuberculosis

Project description:We propose a custom pipeline for combining data from experiments on multispecies studies tested on data from human and bovine peripheral blood mononuclear cellssamples, stimulated with Mycobacterium tuberculosis.

2023-09-15 | GSE218065 | GEO

Characterization of bovine uterine fluid extracellular vesicles proteomic profiles at follicular and luteal phases of the oestrous cycle

Project description:Bovine uterine fluid extracellular vesicles proteomic profile at luteal and follicular phases of the oestrous cycle

2023-07-20 | PXD038002 | Pride

Early response of bovine alveolar macrophages to infection with live and heat-killed Mycobacterium bovis

2010-01-30 | E-GEOD-19237 | biostudies-arrayexpress

Proteomic characterisation of bovine and avian purified protein derivatives and identification of specific antigens for serodiagnosis of bovine tuberculosis

Project description:Bovine purified protein derivative (bPPD) and avian purified protein derivative (aPPD) arewidely used forbovine tuberculosis diagnosis. However, little is known about their qualitative and quantitative characteristics, which makes their standardisation difficult. In addition, bPPDcan give false-positive tuberculosis results because of sequence homologybetween Mycobacterium bovis andM. avium proteins. Here we used proteomics to characterisebPPD, aPPD and an immunopurifiedsubcomplexfrombPPD called P22.

2018-11-22 | PXD007766 | Pride

Quantitative LC-MSMS of mouse lemur liver during early and late winter

Project description:Grey mouse lemurs (Microcebus murinus) are a primate species, which exhibits strong physiological seasonality in response to environmental energetic constraint. They notably accumulate large amounts of lipid reserves during early winter, which are thereafter mobilized during late winter, when food availability is very low. In addition, they develop glucose intolerance in late winter only. To decipher how the hepatic mechanisms may support such metabolic flexibility, we analyzed the liver proteome of captive lemurs, which seasonal regulations of metabolism and reproduction are comparable to their wild counterparts, during the phases of either constitution or use of fat reserves.

2022-05-04 | PXD030198 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data