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Function of ARGONAUTE proteins in the Arabidopsis shoot apical meristem (SAM) [seedling RNA-seq]

ABSTRACT: Function of ARGONAUTE proteins in the Arabidopsis shoot apical meristem (SAM) [seedling RNA-seq]

PROVIDER: PRJNA792338 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17332323_1.fastq.gz	Fastqsanger.gz
	SRR17332323_2.fastq.gz	Fastqsanger.gz
	SRR17332324_1.fastq.gz	Fastqsanger.gz
	SRR17332324_2.fastq.gz	Fastqsanger.gz
	SRR17332325_1.fastq.gz	Fastqsanger.gz

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Function of ARGONAUTE proteins in the Arabidopsis shoot apical meristem (SAM)

Project description:Function of ARGONAUTE proteins in the Arabidopsis shoot apical meristem (SAM)

| PRJNA792336 | ENA

Function of ARGONAUTE proteins in the Arabidopsis shoot apical meristem (SAM).

Project description:Function of ARGONAUTE proteins in the Arabidopsis shoot apical meristem (SAM).

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Function of ARGONAUTE proteins in the Arabidopsis shoot apical meristem (SAM) [seedling RNA-seq]

Project description:In sexually propagating organisms, genetic and epigenetic mutations are evolutionarily relevant only if they occur in the germline and provide inherited information to the next generation. In contrast to most animals, plants are thought to lack an early segregating germline, implying that somatic cells can contribute genetic information to the progeny if they differentiate into meiocytes. Here we demonstrate that two ARGONAUTE proteins, AGO5 and AGO9, are expressed in the reproductive lineage throughout development and mark an early-segregating germline. Furthermore, both AGOs are loaded with dynamic populations of small RNAs derived from highly methylated, pericentromeric, long transposons. Sequencing single nuclei revealed that many of these transposons are highly expressed within a central stem cell domain. This indicates a host-parasite tug of war and specific silencing pathways in plant shoot apical meristems (SAMs). Our results open the path to investigate transposon biology and epigenome dynamics at cellular resolution in the SAM stem cell niche.

2023-09-30 | GSE192609 | GEO

Function of ARGONAUTE proteins in the Arabidopsis shoot apical meristem (SAM) [RNA-seq]

Project description:Function of ARGONAUTE proteins in the Arabidopsis shoot apical meristem (SAM) [RNA-seq]

| PRJNA792339 | ENA

Function of ARGONAUTE proteins in the Arabidopsis shoot apical meristem (SAM) [RIP-seq]

Project description:Function of ARGONAUTE proteins in the Arabidopsis shoot apical meristem (SAM) [RIP-seq]

| PRJNA792340 | ENA

Function of ARGONAUTE proteins in the Arabidopsis shoot apical meristem (SAM) [Bisulfite-Seq]

Project description:Function of ARGONAUTE proteins in the Arabidopsis shoot apical meristem (SAM) [Bisulfite-Seq]

| PRJNA792341 | ENA

Function of ARGONAUTE proteins in the Arabidopsis shoot apical meristem (SAM) [snRNA-seq]

Project description:Function of ARGONAUTE proteins in the Arabidopsis shoot apical meristem (SAM) [snRNA-seq]

| PRJNA792337 | ENA

Function of ARGONAUTE proteins in the Arabidopsis shoot apical meristem (SAM).

2023-10-31 | GSE239462 | GEO

Proteome of rice shoot apical meristem

Project description:DNA methylation is an epigenetic modification that specifies the basic state of pluripotent stem cells and regulates the developmental transition from stem cells to various cell types. In flowering plants, the shoot apical meristem (SAM) contains a pluripotent stem cell population which generates the aerial part of plants including the germ cells. Under appropriate conditions, the SAM undergoes a developmental transition from a leaf-forming vegetative SAM to an inflorescence- and flower-forming reproductive SAM. While SAM characteristics are largely altered in this transition, the complete picture of DNA methylation remains elusive. Here, by analyzing whole-genome DNA methylation of isolated rice SAMs in the vegetative and reproductive stages, we found that methylation at CHH sites is kept high, particularly at transposable elements (TEs), in the vegetative SAM relative to the differentiated leaf, and increases in the reproductive SAM via the RNA-dependent DNA methylation pathway. We also found that half of the TEs that were highly methylated in gametes had already undergone CHH hypermethylation in the SAM. Our results indicate that changes in DNA methylation begin in the SAM long before germ cell differentiation to protect the genome from harmful TEs.

2020-08-14 | PXD020608 | Pride

Molecular Dissection of Pea Shoot Apical Meristem

Project description:Knowledge about an organism’s cell and tissue-specific transcriptional repertoire is essential for understanding the gene regulatory circuits that control key developmental events. The shoot apical meristem (SAM) is responsible for development of all the above ground parts of plants. Our understanding of SAM at the molecular level is far from complete. The present work investigates the global gene expression repertoire of SAMs in the garden pea (Pisum sativum). To this end, 10,346 EST sequences representing 7611 unique genes were generated from pea SAM cDNA libraries. These sequences, together with previously reported ESTs, were used to construct a 12K oligonucleotide array used to identify genes exhibiting differential SAM expression, as compared to the axillary meristem, root apical meristem, and non-meristematic tissues. We identified a number of genes that are predominantly expressed in specific cell layers or domains of the SAM, and thus are likely components of the gene networks involved in stem cell maintenance and initiation of lateral organ primordial cells. In situ hybridization confirmed the spatial localisation of some of these key genes within the SAM. Our data also indicate the diversification of some gene expression patterns and functions in legume crop plants.

2009-10-26 | GSE13451 | GEO

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