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Function of ARGONAUTE proteins in the Arabidopsis shoot apical meristem (SAM) [Bisulfite-Seq]

ABSTRACT: Function of ARGONAUTE proteins in the Arabidopsis shoot apical meristem (SAM) [Bisulfite-Seq]

PROVIDER: PRJNA792341 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17332005.fastq.gz	Fastqsanger.gz
	SRR17332006.fastq.gz	Fastqsanger.gz
	SRR17332007.fastq.gz	Fastqsanger.gz
	SRR17332008.fastq.gz	Fastqsanger.gz
	SRR17332009.fastq.gz	Fastqsanger.gz

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Function of ARGONAUTE proteins in the Arabidopsis shoot apical meristem (SAM)

Project description:Function of ARGONAUTE proteins in the Arabidopsis shoot apical meristem (SAM)

| PRJNA792336 | ENA

Function of ARGONAUTE proteins in the Arabidopsis shoot apical meristem (SAM).

Project description:Function of ARGONAUTE proteins in the Arabidopsis shoot apical meristem (SAM).

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Function of ARGONAUTE proteins in the Arabidopsis shoot apical meristem (SAM) [RNA-seq]

Project description:Function of ARGONAUTE proteins in the Arabidopsis shoot apical meristem (SAM) [RNA-seq]

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Function of ARGONAUTE proteins in the Arabidopsis shoot apical meristem (SAM) [RIP-seq]

Project description:Function of ARGONAUTE proteins in the Arabidopsis shoot apical meristem (SAM) [RIP-seq]

| PRJNA792340 | ENA

Function of ARGONAUTE proteins in the Arabidopsis shoot apical meristem (SAM) [snRNA-seq]

Project description:Function of ARGONAUTE proteins in the Arabidopsis shoot apical meristem (SAM) [snRNA-seq]

| PRJNA792337 | ENA

Microdissection: Transdifferentiation of Root Apical Meristem to Shoot Apical Meristem

Project description:gnp07_regeneome_transdifferenciation - microdissection - Study of the moleculars mecanism during transdifferenciation of Root ApicalMeristem to Shoot Apical Meristem - middle of growth permits to induce transdifferenciation of root apical meristem to shoot apical meristem

2012-06-01 | GSE30259 | GEO

Function of ARGONAUTE proteins in the Arabidopsis shoot apical meristem (SAM) [seedling RNA-seq]

Project description:Function of ARGONAUTE proteins in the Arabidopsis shoot apical meristem (SAM) [seedling RNA-seq]

| PRJNA792338 | ENA

Microdissection: Transdifferentiation of Root Apical Meristem to Shoot Apical Meristem

2012-05-31 | E-GEOD-30259 | biostudies-arrayexpress

Function of ARGONAUTE proteins in the Arabidopsis shoot apical meristem (SAM).

Project description:In sexually propagating organisms, genetic and epigenetic mutations are evolutionarily relevant only if they occur in the germline and provide inherited information to the next generation. In contrast to most animals, plants are thought to lack an early segregating germline, implying that somatic cells can contribute genetic information to the progeny if they differentiate into meiocytes. Here we demonstrate that two ARGONAUTE proteins, AGO5 and AGO9, are expressed in the reproductive lineage throughout development and mark an early-segregating germline. Furthermore, both AGOs are loaded with dynamic populations of small RNAs derived from highly methylated, pericentromeric, long transposons. Sequencing single nuclei revealed that many of these transposons are highly expressed within a central stem cell domain. This indicates a host-parasite tug of war and specific silencing pathways in plant shoot apical meristems (SAMs). Our results open the path to investigate transposon biology and epigenome dynamics at cellular resolution in the SAM stem cell niche.

2023-10-31 | GSE239462 | GEO

Proteome of rice shoot apical meristem

Project description:DNA methylation is an epigenetic modification that specifies the basic state of pluripotent stem cells and regulates the developmental transition from stem cells to various cell types. In flowering plants, the shoot apical meristem (SAM) contains a pluripotent stem cell population which generates the aerial part of plants including the germ cells. Under appropriate conditions, the SAM undergoes a developmental transition from a leaf-forming vegetative SAM to an inflorescence- and flower-forming reproductive SAM. While SAM characteristics are largely altered in this transition, the complete picture of DNA methylation remains elusive. Here, by analyzing whole-genome DNA methylation of isolated rice SAMs in the vegetative and reproductive stages, we found that methylation at CHH sites is kept high, particularly at transposable elements (TEs), in the vegetative SAM relative to the differentiated leaf, and increases in the reproductive SAM via the RNA-dependent DNA methylation pathway. We also found that half of the TEs that were highly methylated in gametes had already undergone CHH hypermethylation in the SAM. Our results indicate that changes in DNA methylation begin in the SAM long before germ cell differentiation to protect the genome from harmful TEs.

2020-08-14 | PXD020608 | Pride

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