Project description:The gut microbiota exerts profound influence on poultry immunity and metabolism through mechanisms that yet need to be elucidated. Here we used conventional and germ-free chickens to explore the influence of the gut microbiota on transcriptomic along the gut-lung axis in poultry. Our results demonstrated a differential regulation of genes associated with innate immunity and metabolism in the caeca of germ-free birds.
Project description:The gut microbiota exerts profound influence on poultry immunity and metabolism through mechanisms that yet need to be elucidated. Here we used conventional and germ-free chickens to explore the influence of the gut microbiota on transcriptomic along the gut-lung axis in poultry. Our results demonstrated a differential regulation of genes associated with innate immunity and metabolism in the lungs of germ-free birds.
Project description:The gut microbiota exerts profound influence on poultry immunity and metabolism through mechanisms that yet need to be elucidated. Here we used conventional and germ-free chickens to explore the influence of the gut microbiota on transcriptomic along the gut-lung axis in poultry. Our results demonstrated a differential regulation of genes associated with innate immunity and metabolism in the spleen of germ-free birds.
Project description:The non-typhoidal Salmonella enterica serotype Heidelberg is a major foodborne pathogen primarily transmitted to humans through contaminated poultry products. Current control measures emphasize novel approaches to mitigate Salmonella Heidelberg colonization in poultry and the contamination of poultry products, thereby reducing its transmission to humans. This study highlight that commensal E. coli 47-1826 can potentially be used to control of S. Heidelberg 18-9079 in poultry
Project description:Salmonella being one of the major infectious diseases in poultry causes considerable economical losses in terms of mortality and morbidity especially in countries which lack effective vaccination programs. Salmonellosis is considered to be most important zoonotic disease which causes considerable foodborne illness that leads to enormous economic loses. To minimize such losses, enhancing disease resistance to different pathogens seems to be a promising strategy. The indigenous chicken, evolved through thousands of years of natural selection, are well adapted to the local climatic conditions with better resistance to diseases. In the present study we investigated liver and spleen transcriptome profile of indigenous (Kashmir faverolla) breed and commercial broiler poultry at day 5 post-inoculation with Salmonella typhimurium using RNA sequencing. The DEGs and pathways identified shall provide potential targets to enhance disease resistance in poultry through successful breeding programmes.
Project description:Salmonella is a common food-borne pathogen that is highly pathogenic and infectious, causing serious harm to livestock breeding and food safety. Uncovering the mechanisms of Salmonella infection and immune evasion can effectively prevent Salmonella contamination of livestock and poultry food. Here, small RNA sequencing results showed that exosomes produced by naïve murine macrophages RAW 264.7 cells contained a unique enrichment of a set of microRNAs (miRNAs) after Salmonella infection.RT-qPCR analysis verified that the tested miRNA (i.e. miR-27a-5p, miR-92a-1-5p and miR-1249-5p) showed similar expression patterns, consistent with small RNA sequencing data. The TargetScan database predicted that the most promising targets for the differentially expressed miRNAs were abundant in the immune system, infectious diseases and signal transduction pathways.
Project description:Early life gut microbiota plays a critical role in gut development, maturation of the immune response and protection against enteric pathogens such as Salmonella. In this study, we investigated how different gut microbiota compositions influence the host transcriptomic signatures and susceptibility to Salmonella infection in chicks.
