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Uncultured actinomycete

ABSTRACT: Diversity of Actinomycete bacteria in nests of fungus-growing ants

PROVIDER: PRJNA79433 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR017133.fastq.gz	Fastqsanger.gz
	SRR017134.fastq.gz	Fastqsanger.gz
	SRR017135.fastq.gz	Fastqsanger.gz
	SRR017136.fastq.gz	Fastqsanger.gz
	SRR017137.fastq.gz	Fastqsanger.gz

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uncultured actinomycete

Project description:uncultured actinomycete overview

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Reverse engineering of industrial pharmaceutical-producing actinomycete strains

Project description:Abstract: Transcript levels in production cultures of wildtype and classically improved strains of the actinomycete bacteria Saccharopolyspora erythraea and Streptomyces fradiae were monitored using microarrays of the sequenced actinomycete S. coelicolor. Sac. erythraea and S. fradiae synthesize the polyketide antibiotics erythromycin and tylosin, respectively, and the classically improved strains contain unknown overproduction mutations. The Sac. erythraea overproducer was found to express the entire 56-kb erythromycin gene cluster several days longer than the wildtype strain. In contrast, the S. fradiae wildtype and overproducer strains expressed the 85-kb tylosin biosynthetic gene cluster similarly, while they expressed several tens of other S. fradiae genes and S. coelicolor homologs differently, including the acyl-CoA dehydrogenase gene aco and the S. coelicolor isobutyryl-CoA mutase homolog icmA. These observations indicated that overproduction mechanisms in classically improved strains can affect both the timing and rate of antibiotic synthesis, and alter the regulation of antibiotic biosynthetic enzymes and enzymes involved in precursor metabolism. This SuperSeries is composed of the SubSeries listed below.

2006-03-08 | GSE4395 | GEO

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Streptomyces sp. CB0028 and Escovopsis sp. CBACRO424

Project description:Fungus-growing ants microbial interaction of Streptomyces sp. and Escovopsis sp. through molecular networking and MALDI imaging

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Imaging with Mass Spectrometry of Bacteria on the Exoskeleton of Fungus-Growing Ants

Project description:Mass spectrometry imaging is a powerful analytical technique for detecting and determining spatial distributions of molecules within a sample. Typically, mass spectrometry imaging is limited to the analysis of thin tissue sections taken from the middle of a sample. In this work, we present a mass spectrometry imaging method for the detection of compounds produced by bacteria on the outside surface of ant exoskeletons in response to pathogen exposure. Fungus-growing ants have a specialized mutualism with Pseudonocardia, a bacterium that lives on the ants’ exoskeletons and helps protect their fungal garden food source from harmful pathogens. The developed method allows for visualization of bacterial-derived compounds on the ant exoskeleton. This method demonstrates the capability to detect compounds that are specifically localized to the bacterial patch on ant exoskeletons, shows good reproducibility across individual ants, and achieves accurate mass measurements within 5 ppm error when using a high-resolution, accurate-mass mass spectrometer.

2018-07-26 | MTBLS471 | MetaboLights

Project description:Bacterial communities of A.echinatior fungus-growing ants Targeted loci environmental

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Lichen and actinomycete samples

Project description:Mixed lichen and actinomycete samples collected in NZ

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From plants to ants: Evaluating lipidomic changes in leaf-cutter ant fungus gardens

Project description:Leaf-cutter ants use fresh plant material to cultivate an obligate mutualistic fungus Leucoagaricus gongylophorus in specialized fungus gardens to access and transform nutrients from plant biomass that would otherwise be unavailable to the ants. Here, we evaluated the lipidomic differences between the leaves feeding the gardens, gongylidia produced by the fungus to feed the ants, and spatially-resolve regions of the fungus garden at initial to advanced stages of leaf degradation.

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