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The impact of lactate and indole-3-propionate (IPA) on metabolic and epigenetic state of Th17 cells.

ABSTRACT: The impact of lactate and indole-3-propionate (IPA) on metabolic and epigenetic state of Th17 cells.

PROVIDER: PRJNA795954 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17524930.fastq.gz	Fastqsanger.gz
	SRR17524931.fastq.gz	Fastqsanger.gz
	SRR17524932.fastq.gz	Fastqsanger.gz
	SRR17524933.fastq.gz	Fastqsanger.gz
	SRR17524934.fastq.gz	Fastqsanger.gz

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Similar Datasets

The impact of lactate and indole-3-propionate (IPA) on metabolic and epigenetic state of Th17 cells.

Project description:Purpose: Although glycolysis-derived lactate and bacterial product IPA are active metabolites, there is still a lack of understanding of their influence on metabolic and epigentic status of T cells. The aim of this study was to shed light on possible effects of lactate and IPA on function of T cells, including production of cytokines and regulation of trenscriptional factors. Method: Naive mice (8-12-week-old female mice) were sacrificed and CD4+ T cells were purified from spleens and lymph nodes. T lymphocytes were then treated with lactate or IPA for two days. To ensure that gene signatures were derived from T cells and not from contaminating APCs or B cells, T cell isolation kit from Miltenyi Biotec was used (purity over 95 %). Results: The analysis of differential expressed genes of lactate- and IPA-treated Th17 cells revealed multiple effects of extracellular lactate and bacterial IPA on the transcriptional profiles. Lactate induced expression of diverse genes associated with transcriptional signature of regulatory T cells. Conclusion: This study demonstrated that extracellular lactate and microbial metabolite IPA influence transcriptional programs in murine Th17 cells.

2022-11-25 | GSE193358 | GEO

Pelosinus strain:IPA-1

Project description:Pelosinus sp. IPA-1

| PRJDB14839 | ENA

ChIP-seq of H3K18la in murine Th17-differentiated T cells

Project description:Increased lactate levels in the tissue microenvironment are a well-known feature of chronic inflammation. However, the role of lactate in regulating T cell function remains controversial. We here demonstrate that extracellular lactate predominantly induces deregulation of the Th17-specific gene expression program by modulating metabolic and epigenetic status of Th17 cells. Following lactate treatment, Th17 cells significantly reduced their IL-17A production and upregulated Foxp3 expression through ROS-driven IL-2 secretion. Moreover, we observed a broad pattern of histone lactylation at various genes in CD4+ T cells, with particularly highly enriched lactylation of histone H3 at the Foxp3 promoter regions in lactate-treated Th17 cells. These results indicate that lactate is capable of reprogramming pro-inflammatory T cell phenotype into regulatory T cells. Moreover, we show that high lactate concentrations suppress the Th17 pathogenicity during intestinal inflammation. Collectively, these findings have a potential therapeutic value for development of novel clinical strategies to target inflammatory and autoimmune diseases.

2022-11-25 | GSE208727 | GEO

Gut-derived indole propionic acid alleviates liver fibrosis by modulating pro-fibrogenic macrophage phenotype and biological function

Project description:Recently, gut metabolites have been recognized to play significant roles in liver diseases via the gut-liver axis. In this study, we investigate the regulatory effects of the tryptophan metabolite, indole-3-propionic acid (IPA), on immune cells during liver fibrogenesis using single-cell RNA sequencing (scRNA-seq).

2024-09-20 | GSE277148 | GEO

Kentucky assay: Indole

Project description:Kentucky assay: Indole

| PRJNA610533 | ENA

RNA sequencing of L4-L6 mouse Dorsal Root Ganglia 3 days post sciatic nerve crush and naive following 10 days of Indole-3-propionic acid treatment

Project description:Purpose: compare the response of DRG neurons to indole-3-propionic acid treatment before and after sciatic nerve injury Results: we found that IPA induced transcription changes before and after injury inside the DRG tissue. In particular expression of immune system related genes

2022-01-01 | GSE161342 | GEO

Salmonella wild type in LB, 2mM indole until OD1 and 2mM indole for 15 minutes

Project description:Transcriptional profiling of Salmonella Typhimurium SL1344 wild type grown to OD600=1 in LB alone or in LB supplemented with 2mM Indole, or with 2mM indole for 15 min (called shock). The goal is to determine Salmonella response to indole at different time points.

2013-01-30 | GSE39760 | GEO

Investigation on the anaerobic propionate degradation by Escherichia coli K12

Project description:Propionate is an abundant carboxylic acid in nature. Microorganisms metabolize propionate aerobically via the 2-methylcitrate pathway. This pathway depends on a series of three reactions in the citric acid cycle that leads to the conversion of succinate to oxaloacetate. Interestingly, the gamma-proteobacterium Escherichia coli can use propionate as a carbon and electron source under oxic but not under anoxic conditions. The typical downregulation of the citric acid cycle under anoxic conditions is only partially responsible for the inability to use propionate under anoxic conditions since an arcA mutant shows very limited growth on propionate. RT-PCR and transcriptomic analysis revealed a post-transcriptional regulation of the prp-genecluster encoding the necessary enzymes for propionate metabolism. The polycistronic mRNA was hydrolyzed in the 3`-5` direction under anoxic conditions. This regulatory strategy is highly constructive because the last gene of the operon encodes the first enzyme of the propionate metabolism. Further analysis revealed that RNase R catalyzes the hydrolysis of the prp transcripts. Consequently, an rnr-deletion strain could metabolize propionate under anoxic conditions. To the best of our knowledge, this is the first study describing the influence of RNase R on the anaerobic metabolism of E. coli.

2017-12-14 | GSE78814 | GEO

Pseudomonas putida KT2440 : Control vs. indole or ampicillin or indole plus ampicillin treated

Project description:Transcriptional profiling of P. putida KT2440 cells comparing untreated cells with 1 mM indole or 50 μg/ml ampicillin or 1 mM indole plus 50 μg/ml ampicillin treated cells

2016-09-10 | GSE86617 | GEO

CIP2A negatively regulates Th17 differentiation

Project description:Interleukin 17 (IL-17) producing T helper 17 (Th17) cells are critical drivers of pathogenesis in a variety of autoimmune and inflammatory diseases. Strategies to mitigate excessive Th17 response thus remain an attractive target for immunotherapies. Here we report that Cancerous Inhibitor of Protein Phosphatase 2A (CIP2A) regulates IL-17 production by Th17 cells in human and mouse. Using CIP2A knock-out (KO) mice and siRNA-mediated CIP2A silencing in human primary CD4+ T cells, we demonstrated that CIP2A silencing results in a significant increase in IL-17 production. Interestingly, CIP2A deficient Th17 cells were characterized by increased strength and duration of STAT3 (Y705) phosphorylation. Genome-wide gene expression profile as well as the p-STAT3 (Y705) interactome of CIP2A deficient Th17 cells identified that CIP2A regulates the strength of the interaction between Acylglycerol kinase (AGK) and STAT3, and thereby, modulates STAT3 phosphorylation as well as expression of IL-17 in Th17 cells. Our results uncover the physiological function of CIP2A in Th17 cells and provides new opportunities for therapeutic intervention in Th17 cell mediated diseases.

2020-05-26 | PXD010612 | Pride

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