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Microtus ochrogaster

ABSTRACT: Transcriptome sequencing of Prairie vole

PROVIDER: PRJNA79609 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR054642.fastq.gz	Fastqsanger.gz
	SRR054643.fastq.gz	Fastqsanger.gz
	SRR054644.fastq.gz	Fastqsanger.gz
	SRR054645.fastq.gz	Fastqsanger.gz
	SRR054646.fastq.gz	Fastqsanger.gz

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Genome wide identification of enhancer regions in the retrosplenial cortex of the prairie vole, Microtus ochrogaster.

2015-12-11 | GSE73670 | GEO

Genome wide identification of enhancer regions in the retrosplenial cortex of the prairie vole, Microtus ochrogaster.

Project description:We use ChIP-seq targeting histone 3 lysine 4 mono-methylation (H3K4me1) to identify putative enhancer sites genome-wide, in the retrosplenial cortex of adult prairie vole males. ChIP samples were generated by targeting a known enhancer mark (H3K4me1) in chromatin extracted from the retrosplenial cortex of 8 males. Illumina libraries were prepared from ChIP and INPUT DNA and sequenced on Illimuna HiSeq 2500 platform.

2015-12-11 | E-GEOD-73670 | biostudies-arrayexpress

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Project description:The prairie vole is notable for its life-long pair-bonds

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Project description:Becoming monogamous quickly: Adaptive evolution of a pallidal enhancer at the prairie vole Avpr1a locus

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Microtus ochrogaster

Project description:Genome wide identification of enhancer regions in the retrosplenial cortex of the prairie vole, Microtus ochrogaster.

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Project description:Microtus ochrogaster Raw sequence reads

| PRJNA382962 | ENA

Fecal microbiota in the female prairie vole (Microtus ochrogaster).

Project description:We examined the fecal microbiota of female prairie voles. This species is socially and, likely, sexually monogamous, and thus serves as a valuable model in which to examine the interaction between the microbiota-gut-brain axis and social behavior. At present, little is known about the gastrointestinal microbiota of prairie voles; therefore, we performed a first characterization of the fecal microbiota using 16S rRNA gene amplicon sequencing. Semiconductor sequencing technology on an Ion Torrent PGM platform was used to assess the composition of fecal microbiotas from twelve female prairie voles. Following quality filtering, 1,017,756 sequencing reads were classified from phylum to genus level. At the phylum level, Firmicutes, Bacteroidetes, and Saccharibacteria were the predominant taxa, while the Bacteriodales, Erysipelotrichaceae, Ruminococcaceae, and Lachnospiraceae contributed the most dominant microbial groups and genera. Microbial community membership was most similar between vole sibling pairs, but consideration of taxon abundances weakened these associations. The interdependence of host factors such as genetics and behavior with the gastrointestinal microbiota is likely to be particularly pronounced in prairie voles. Our pilot characterization of the prairie vole intestinal microbiota revealed a microbial community composition remarkably consistent with the monogastric alimentary system of these rodents and their diet rich in complex plant carbohydrates. The highly social nature of these animals poses specific challenges to microbiome analyses that nonetheless are valuable for advancing research on the microbiota-gut-brain-behavior axis. Our study provides an important basis for future microbiome research in this emerging model organism for studying social behavior.

| S-EPMC5868765 | biostudies-literature

Lactobacilli with probiotic potential in the prairie vole (Microtus ochrogaster).

Project description:BackgroundRecent research suggests integration of the intestinal microbiota in gut-brain communication which could lead to new approaches to treat neurological disorders. The highly social prairie voles are an excellent model system to study the effects of environmental factors on social behavior. For future studies on the role of probiotics in ameliorating disorders with social withdrawal symptoms, we report the characterization of intestinal Lactobacillus isolates with probiotic potential from voles.Methods and results30 bacterial strains were isolated from the vole intestine and found to be distinct but closely related to Lactobacillus johnsonii using 16S rRNA gene sequencing and Random Amplification of Polymorphic DNA fingerprinting. In vitro characterizations including acid and bile tolerance, antimicrobial effects, antibiotic susceptibility, and adherence to intestinal epithelial cells were performed to assess the probiotic potential of selected strains. Since previous studies revealed that mercury ingestion triggers social deficits in voles, mercury resistance of the probiotic candidates was evaluated which could be an important factor in preventing/treating these behavioral changes.ConclusionsThis study demonstrates that lactobacilli with probiotic potential are present in the vole intestine. The Lactobacillus isolates identified in this study will provide a basis for the investigation of probiotic effects in the vole behavioral model system.

| S-EPMC4696317 | biostudies-literature

Microtus ochrogaster isolate:LTLLF

Project description:Microtus ochrogaster isolate:LTLLF Genome sequencing and assembly

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North American Microtus

Project description:Sequencing vole sex chromosomes Raw sequence reads

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