Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Expression data from MCF7 cells treated with hits from screening cytotoxic michael acceptors

ABSTRACT: Expression data from MCF7 cells treated with hits from screening cytotoxic michael acceptors

PROVIDER: PRJNA796574 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Expression data from MCF7 cells treated with hits from screening cytotoxic michael acceptors

Project description:We used a chemical library to screen for cytotoxic compounds with Michael acceptor groups that induce a cellular response characteristic of proteasome inhibition. We then determined the differentially expressed genes in MCF7 cells following treatment with each of the identified hits This dataset includes expression data from MCF7 cells treated with either DMSO or one of the treatment compounds

2022-02-17 | GSE193542 | GEO

Elavl HITS-CLIP

Project description:Elavl HITS-CLIP

| PRJNA407981 | ENA

SRSF1 HITS-CLIP

Project description:SRSF1 HITS-CLIP

| PRJNA541847 | ENA

Methylomes of Geobacter sulfurreducens Grown on Different Terminal Electron Acceptors

Project description:The Geobacter species evolved respiratory versatility to utilize a wide range of terminal electron acceptors. To explore this adaptive mechanism, Fe(III) citrate, hydrous ferric oxide, and fumarate were selected as electron acceptors, and the methylome and metabolome of Geobacter sulfurreducens PCA grown on each electron acceptor were investigated via third-generation, single-molecule real-time DNA sequencing.Results showed that the patterns of 4-methylcytosine (m4C) and 6-methyladenine (m6A) modification were all varied in different electron acceptor cultures. Moreover, genes (e.g., GSU0466 and GSU1467) with low expression levels generally had high methylation levels. These findings suggest that m4C and m6A modifications play a role in the adaption of G. sulfurreducens to diverse electron acceptors, and DNA methylation may be involved in the adaption mainly via gene expression regulation.

2021-08-31 | GSE157224 | GEO

Gene expression profiling of antigen-specific T cells upon CCR9 knockdown in MCF7 cells.

Project description:Tumor cells are known to express immune-inhibitory ligands on their surface to mediate resistance against cytotoxic T lymphocytes. Using a high-throughput RNAi screening assay, we identified CCR9 as a potent tumor-specific immune checkpoint molecule that inhibited T cell-mediated tumor lysis. To understand the mode of CCR9-mediated immune resistance, MCF7 tumor cells were transfected with control or CCR9-specific siRNAs for 72 hours and co-cultured with survivin antigen-specific T cells for an additional 12 hours. T cells were then purified from the co-culture and total RNA was isolated for gene expression analysis.

2015-02-19 | E-MTAB-3244 | biostudies-arrayexpress

HITS-CLIP of SMN

Project description:HITS-CLIP of SMN

| PRJNA433653 | ENA

PCP degradation under different electron acceptors

Project description:PCP degradation under different electron acceptors Metagenome

| PRJNA411964 | ENA

YBX1 HITS-CLIP

Project description:In order to identify YBX1 binding sites on endogenous RNA, we performed HITS-CLIP on endogenous YBX1 We used a previously published method to perform HITS-CLIP on endogenous YBX1 (Licatalosi D, et al. 2008, Nature 456:464-U22)

2015-05-08 | E-GEOD-63604 | biostudies-arrayexpress

PCPTC screening

Project description:Primary cultures of patient tumor cells (PCPTC) were used in a cell-based cytotoxicity screen. Microarray-based mRNA profiling was used to identify the mechanism-of-action for the small molecule VLX 50. MCF7 cells were treated with the PCPTC screening hit VLX 50 or DMSO control for 6h prior to RNA isolation. One sample per treatment. Data were analyzed using both MAS5.0 and RMA.

2011-09-08 | E-GEOD-24027 | biostudies-arrayexpress

TARBP2 HITS-CLIP

Project description:In order to identify TARBP2 binding sites on endogenous RNA, we performed HITS-CLIP on a myc-tagged TARBP2 expressing cell-line (transient transfection) Cells were transfected with tagged TARBP2 vector (Origene) and 48-hr post-transfection, they were subjected to the HITS-CLIP procedure (Licatalosi D, et al. 2008, Nature 456:464-U22)

2014-07-24 | E-GEOD-49648 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data