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Gene differential expression of autophagy-related gene ablation in 562 vascular tumor cells

ABSTRACT: Gene differential expression of autophagy-related gene ablation in 562 vascular tumor cells

PROVIDER: PRJNA796605 | ENA |

REPOSITORIES: ENA

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Gene differential expression of autophagy-related gene ablation in 562 vascular tumor cells

Project description:To explore the mechanisms of autophagy in the regulation of vascular tumour cells, we generated three autophagy-related genes, Fip200, Atg5 and Atg7, knockout vascular tumor cells in 562 cell line by CRISPR-Cas9-based sgRNAs. Three independent replicates of mRNA samples were prepared from each KO cells and subjected to RNA-sequencing in comparison to three samples from control 562 cells. We examined changes in gene expression by transcriptional profiling of Fip200 KO, Atg5 KO, and Atg7 KO cells, compared to control 562 cells respectively.

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Project description:The decline of endothelial autophagy is closely related to vascular senescence and disease, although the molecular mechanisms connecting these outcomes in vascular endothelial cells (VECs) remain unclear. Here, we identify a crucial role for CD44, a multifunctional adhesion molecule, in controlling autophagy and aging in VECs. The CD44 intercellular domain (CD44ICD) negatively regulates autophagy by reducing PIK3R4 and PIK3C3 levels and disrupting STAT3-dependent PtdIns3K complexes. CD44 and its homologue clec-31 are increased in aging vascular endothelium and Caenorhabditis elegans, respectively, suggesting that an age-dependent increase in CD44 induces autophagy decline and aging phenotypes. Accordingly, CD44 knockdown ameliorates age-associated phenotypes in VECs. The endothelium-specific CD44ICD knock-in mouse is shorter-lived, with VECs exhibiting obvious premature aging characteristics associated with decreased basal autophagy. Autophagy activation suppresses the premature aging of human and mouse VECs overexpressing CD44ICD, function conserved in the CD44 homologue clec-31 in C. elegans. Our work describes a mechanism coordinated by CD44 function bridging autophagy decline and aging.

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Project description:We analyzed the global effect of c-Myb knockdown by sequencing the transcriptomes of K-562 cells transfected with control siRNA and si2992 (MYB knockdown), as well as K-562 cells stably expressing TY-tagged wild type c-Myb and c-Myb D152V transfected with si2992

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Coemansia sp. RSA 562 isolate:RSA 562

Project description:Coemansia sp. RSA 562 Resequencing

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Project description:Differential complementary positional proteomics of human granzyme B treated K-562 lysates

2010-05-19 | PRD000229 | Pride

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Project description:K-562 cell lines

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CD44 connects autophagy decline and aging in the vascular endothelium

Project description:CD44 connects autophagy decline and aging in the vascular endothelium

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Lhx8 ablation leads to massive autophagy of mouse oocytes associated with DNA damage

Project description:Lhx8 ablation leads to massive autophagy of mouse oocytes associated with DNA damage

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gene expression of erythromyeloid K-562 cells

Project description:K-562 cells were cultured in standard growth conditions and used to produce five distinct cell cultures: wild type, FHC-silenced,wild type hemin-treated, FHC-silenced hemin-treated. Then the cells were lysed and RNA was extracted using TRIzol method.Total RNA were fluorescently labelled, amplified and hybridized in triplicate for 18 hours on Illumina HumanHT12 v.4.0 Expression BeadChips and after scanning, analysis was performed with GenomeStudio v.2010.3 software, for quality control and mRNA expression analysis.

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