Project description:Insulin degrading enzyme (IDE) is a major enzyme responsible for insulin degradation in the liver. The modulation of insulin degrading enzyme activity is hypothesized to be a link between T2DM and liver cancer. Results provide insight into role of IDE in proliferation and other cell functions.
Project description:Insulin degrading enzyme (IDE) is a major enzyme responsible for insulin degradation in the liver. The modulation of insulin degrading enzyme activity is hypothesized to be a link between T2DM and liver cancer. Results provide insight into role of IDE in proliferation and other cell functions. HepG2 cells were transfected with 96nM siRNA for IDE or AllStars Negative Control siRNA (Qiagen) using Lipofectamine 2000 (Invitrogen). 16 h after transfection, cells were treated with 10 nM insulin (Sigma Aldrich) or vehicle for 24 h in serum starvation condition. Total RNA was extracted. For each of the 4 conditions, 3 biological replicates were included.
Project description:To explore the effect of transcription factor POX01387 on transcription levels of plant-biomass-degrading enzyme-encoding genes in Penicillium oxalicum
Project description:To explore the effect of POX03070 on transcription levels of plant-biomass-degrading enzyme-encoding genes in Penicillium oxalicum,and the interaction between POX03070 and PoxCxrA in transcriptional level.
Project description:Transcriptomic profiling and real-time quantitative reverse transcription-PCR analyses revealed that TP05746 dynamically regulated the expression of genes encoding major PBDEs. Furthermore, in vitro binding experiments confirmed that TP05746 directly bound to the promoter regions of these major enzyme genes. Transcription factors mediated the regulation of plant-biomass-degrading enzyme (PBDE) gene expression in Talaromyces pinophilus.