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ICLIP of endogenous PURA in neuronal precursor cells

ABSTRACT: iCLIP of endogenous PURA in neuronal precursor cells

PROVIDER: PRJNA798376 | ENA |

REPOSITORIES: ENA

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iCLIP of endogenous PURA in neuronal precursor cells

Project description:The protein PURA binds to DNA and RNA and has been suggested to be implicated in different cellular functions. Here, we show that PURA predominantly resides in the cytoplasm, where it binds to a large set of transcripts.

2022-12-12 | GSE193902 | GEO

iCLIP of endogenous PURA in HeLa cells

Project description:iCLIP of endogenous PURA in HeLa cells

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iCLIP of endogenous PURA in HeLa cells

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iCLIP of FLAG-PURA overexpression with anti-PURA12D11 antibody in HeLa cells

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iCLIP of FLAG-PURA overexpression with anti-FLAG antibody in HeLa cells

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iCLIP of FLAG-PURA overexpression with anti-FLAG antibody in HeLa cells

Project description:iCLIP of FLAG-PURA overexpression with anti-FLAG antibody in HeLa cells

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iCLIP of FLAG-PURA overexpression with anti-PURA12D11 antibody in HeLa cells

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RNAseq of PURA knockdown in HeLa cells

Project description:Here, we show that PURA predominantly resides in the cytoplasm, where it binds to a large set of transcripts. Many of these transcripts change abundance in response to PURA depletion, in parts reflected in altered protein expression levels. A closer inspection of the regulated proteins indicates a role of PURA in immune responses, mitochondrial function, autophagy and processing (P)-body activity.

2022-12-12 | GSE193900 | GEO

Depletion of the RNA-binding protein PURA triggers changes in posttranscriptional gene regulation and loss of P-bodies

Project description:The RNA-binding protein PURA has been implicated in the rare, monogenetic, neurodevelopmental disorder PURA Syndrome. PURA binds both DNA and RNA and has been associated with various cellular functions. Only little is known about its main cellular functions and the molecular pathways affected upon PURA depletion. Here, we show that PURA is predominantly located in the cytoplasm, where it binds to thousands of mRNAs. Many of these transcripts change abundance in response to PURA depletion. The encoded proteins suggest a role for PURA in immune responses, mitochondrial function, autophagy and processing (P)-body activity. Intriguingly, reduced PURA levels decrease the expression of the integral P-body components LSM14A and DDX6, and strongly affect P-body formation in human cells. Furthermore, PURA knockdown results in stabilization of P-body-enriched transcripts, whereas other mRNAs decrease. Hence, reduced PURA levels, as reported in patients with PURA Syndrome, influence the formation and composition of this phase-separated RNA processing machinery. Our study on PURA provides a blueprint for the comprehensive understanding of the tight connection between P-body-associated RNA regulation and neurodevelopmental disorders.

2022-12-19 | PXD030266 | Pride

Nuclear miCLIP and Nuclear NKAP iCLIP

Project description:To identify m6A sites on endogenous nuclear RNAs, we performed miCLIP to identify m6A sites in PANC-1 cells. To identify NKAP binding sites on endogenous nuclear RNAs, we performed iCLIP for flag-tag NKAP to analyze the nuclear RNA binding with NKAP in the same cells.

2019-03-24 | GSE116425 | GEO

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