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RNA-seq analyses of doxycycline-induced PROX1-exprressing cells in human colon cancer spheroids

ABSTRACT: RNA-seq analyses of doxycycline-induced PROX1-exprressing cells in human colon cancer spheroids

PROVIDER: PRJNA798429 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17657130_1.fastq.gz	Fastqsanger.gz
	SRR17657130_2.fastq.gz	Fastqsanger.gz
	SRR17657131_1.fastq.gz	Fastqsanger.gz
	SRR17657131_2.fastq.gz	Fastqsanger.gz
	SRR17657132_1.fastq.gz	Fastqsanger.gz

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RNA-seq analyses of doxycycline-induced PROX1-exprressing cells in human colon cancer spheroids

Project description:We performed RNA-seq analyses of the human cancer spheroids expressing PROX1 induced by doxycycline treatment.

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PROX1 mediates chemoresistance-associated recurrence via maintenance of quiescent colon cancer stem cells

Project description:Cancer stem cells (CSCs) are profoundly associated with refractory nature of cancer. A quiescent population of CSCs is responsible for tumorigenesis and chemoresistance in leukemia, whereas neither the presence nor clinical importance of the quiescent CSCs is clearly established in solid tumors. In colon cancer, LGR5 is regarded as a functional marker of CSCs, but heterogeneity among LGR5+ cells was not clearly defined. Here we stratified LGR5+ cells by single-cell gene expression analyses and revealed that a tumorigenic population of mouse LGR5+ cells resides in a quiescent state. We identified 23 signature genes that were uniquely expressed in the quiescent CSCs of mouse tumors, and found that 7 of them were also specifically expressed in a quiescent population of LGR5+ cells in human colon xenograft tumors. Among them, PROX1 was expressed in invasive fronts of colon tumors. PROX1 was induced by TCF1 (TCF7), and the TCF1-mediated PROX1 expression was responsible for not only the maintenance of quiescence but also chemoresistance of colon cancer organoids. Knockout of PROX1 in patient-derived xenograft tumors resulted in inhibition of tumor recurrence after chemotherapeutic treatment. Our data underscore the therapeutic importance of a quiescent CSC population in colon cancer.

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Transcriptional profile changes by Prox1 expression in thyroid cancer cells

Project description:Human thyroid cancer cell line BCPAP was engineered for doxycyclin-mediated inducible expressoin of Prox1 and next generation sequcing was performed to study the transcriptional regulation by Prox1 BCPAP cells were infected with rTTA2 lentivirus. The resulting cell populations were then stably transfected either with pIRES-hygromycin vector (Clontech) to generate BCPAP-Ctr cells, or with Flag-PROX1-IRES-hygromycin vector to generate BCPAP-Prx cells. PROX1 expression was induced or not in BCPAP-Ctr and BCPAP-Prx cells with 0.5 Âµg/ml doxycycline (Dox) for 48 hours and the total RNA samples were collected for sequencing.

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