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Comparison of monoculture to coculture colon cancer cell-line generated spheroids

ABSTRACT: Chromatogram library generated of pooled sample. Coculture spheroids formed from fibroblast and colon cancer cell lines, and monoculture spheroids formed from the colon cancer cell line HCT116.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Amanda Hummon

PROVIDER: MSV000093395 | MassIVE | Mon Nov 13 20:41:00 GMT 2023

SECONDARY ACCESSION(S): PXD046921

REPOSITORIES: MassIVE

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Publications

Gas-Phase Fractionation Data-Independent Acquisition Analysis of 3D Cocultured Spheroid Tumor Model Reveals Altered Translational Processes and Signaling Using Proteomics.

Shannon Ariana E AE Boos Claire E CE Searle Brian C BC Hummon Amanda B AB

Journal of proteome research 20240227 8

Colorectal cancer (CRC) contains considerable heterogeneity; therefore, models of the disease must also reflect the multifarious components. Compared to traditional 2D models, 3D cellular models, such as tumor spheroids, have the utility to determine the drug efficacy of potential therapeutics. Monoculture spheroids are well-known to recapitulate gene expression, cell signaling, and pathophysiological gradients of avascularized tumors. However, they fail to mimic the stromal cell influence prese ...[more]

PMID: 38412258

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