Project description:Npas4 CUT&Tag dataset, Adult male WT C57BL/6J mice underwent discriminative fear conditioning and immediately injected saline. 90 minutes after fear conditioning, The amygdala tissue was extracted for further experiment. Npas4 CUT&Tag was performed to elucidate possible downstream targets which contributes regulation of fear expression during fear retreival.
Project description:We performed single-cell RNA-seq of full, microdissected and dissociated mouse amygdala, 2h, 8h or 24h after tone-cued fear conditioning (CFC), and 2h after recall (exposure to CS only, 24h post-CFC), and naive homecage controls.
Project description:Analysis of amygdala RNA expression 2 hours after auditory fear conditioning in mice with and without previous exposure to acute immobilization stress Total RNA from three groups was obtained: 1) Home Cage (HC) 2) Fear Conditioning (FC) 3) Immobilization (IMO) + FC
Project description:Fear conditioning in rats leads to long term memory (LTM) formation. A central neural substrate for LTM is the basolateral amygdala. We sought the expression changes specific to LTM at 6h following anesthesia with isoflurane (a general anesthetic and an effective amnestic agent), following pain (a component of conditioning), and following conditioning in presence and absence of isoflurane.
Project description:Fear conditioning in rats leads to long term memory (LTM) formation. A central neural substrate for LTM is the basolateral amygdala. We sought the expression changes specific to LTM at 6h following anesthesia with isoflurane (a general anesthetic and an effective amnestic agent), following pain (a component of conditioning), and following conditioning in presence and absence of isoflurane. Keywords = anesthesia, amygdala, LTM, Rampil, Isoflurane, fear, memory Keywords: other
Project description:Analysis of amygdala RNA expression after auditory fear conditioning in mice. Total RNA from three groups was obtained: 1) Home Cage (HC) 2) 30 minutes after Fear Conditioning (FC) 3) 2 hours after FC.
Project description:Patients with psychiatric disorders show higher serum phospholipase A2 (PLA2) activity, however the relationship between PLA2 and psychiatric disorders has been poorly understood. It has been known that 129S1/SvImJ (S1) mice exhibit impaired fear extinction, one of the major symptoms of post-traumatic stress disorder (PTSD). In fear conditioning, sensory and somatic inputs arrive in the basolateral amygdala (BLA) and relay to the medial division of the central amygdala (CEm), the final output in the amygdala projecting to fear response-generating areas. Here we found that S1 mice showed increased expression of PLA2 mRNA in the BLA and CEm compared to control C57BL/6 (B6) mice. Basal inhibitory transmission of CEm neurons was decreased in S1 mice and acute inhibition of PLA2 successfully elevated the basal inhibitory transmission levels in the CEm both in naïve B6 and S1 mice. PLA2 inhibition also weakened BLA to CEm synapses in S1 mice after fear extinction, thereby leading the synaptic responses from CEm while stimulating BLA back to the levels comparable to B6. Ultimately, the selective infusion of PLA2 inhibitors into the CEm enhanced fear extinction in S1 mice. Our data suggest that PLA2 activity modulates inhibitory transmission of the CEm and fear extinction, thus possibly serves as a key molecular mediator of impaired fear extinction in PTSD.
Project description:This project examined sex differences in the role of K48 polyubiquitination in the amygdala during the indirect acquisition (observing another animal undergo fear conditioning) of an auditory fear memory formation in male and female rats.