Project description:Npas4 CUT&Tag dataset, Adult male WT C57BL/6J mice underwent discriminative fear conditioning and immediately injected saline. 90 minutes after fear conditioning, The amygdala tissue was extracted for further experiment. Npas4 CUT&Tag was performed to elucidate possible downstream targets which contributes regulation of fear expression during fear retreival.
Project description:We performed single-cell RNA-seq of full, microdissected and dissociated mouse amygdala, 2h, 8h or 24h after tone-cued fear conditioning (CFC), and 2h after recall (exposure to CS only, 24h post-CFC), and naive homecage controls.
Project description:Analysis of amygdala RNA expression 2 hours after auditory fear conditioning in mice with and without previous exposure to acute immobilization stress Total RNA from three groups was obtained: 1) Home Cage (HC) 2) Fear Conditioning (FC) 3) Immobilization (IMO) + FC
Project description:Fear conditioning in rats leads to long term memory (LTM) formation. A central neural substrate for LTM is the basolateral amygdala. We sought the expression changes specific to LTM at 6h following anesthesia with isoflurane (a general anesthetic and an effective amnestic agent), following pain (a component of conditioning), and following conditioning in presence and absence of isoflurane.
Project description:Fear conditioning in rats leads to long term memory (LTM) formation. A central neural substrate for LTM is the basolateral amygdala. We sought the expression changes specific to LTM at 6h following anesthesia with isoflurane (a general anesthetic and an effective amnestic agent), following pain (a component of conditioning), and following conditioning in presence and absence of isoflurane. Keywords = anesthesia, amygdala, LTM, Rampil, Isoflurane, fear, memory Keywords: other
Project description:Analysis of amygdala RNA expression after auditory fear conditioning in mice. Total RNA from three groups was obtained: 1) Home Cage (HC) 2) 30 minutes after Fear Conditioning (FC) 3) 2 hours after FC.
Project description:This dataset constitutes the first Sono-Seq study of chromatin accessibility following contextual fear conditioning in the mouse hippocampus.
Project description:This dataset constitutes the first RNA-seq study of gene expression following contextual fear conditioning in the mouse hippocampus.