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RNAseq analysis of Hep3B cells upon overexpression of LINE1 retrotransposon encoded protein L1orf1p

ABSTRACT: RNAseq analysis of Hep3B cells upon overexpression of LINE1 retrotransposon encoded protein L1orf1p

PROVIDER: PRJNA800007 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17717023.fastq.gz	Fastqsanger.gz
	SRR17717024.fastq.gz	Fastqsanger.gz
	SRR17717025.fastq.gz	Fastqsanger.gz
	SRR17717026.fastq.gz	Fastqsanger.gz
	SRR17717027.fastq.gz	Fastqsanger.gz

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RNAseq analysis of Hep3B cells upon overexpression of LINE1 retrotransposon encoded protein L1orf1p

Project description:L1 retrotransposons are known to get activated during hepatocarcinogenesis. We have developed inducible (TET-ON) overexpression of L1orf1 using piggybac vector in Hep3B cell line and a control TET-ON-empty cell line was also generated. Influence of L1orf1 overexpression on overall cellular transcritome was then analysed by RNAseq followed by pathway analysis. Overall, a number of key oncogeneic pathways are dysregulated upon L1orf1 overexpression is observed.

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