Ontology highlight
ABSTRACT:
PROVIDER: PRJNA802541 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| Other | ||||
| SRR17861134.fastq.gz | Fastqsanger.gz | |||
| SRR17861135.fastq.gz | Fastqsanger.gz | |||
| SRR17861136.fastq.gz | Fastqsanger.gz | |||
| SRR17861137.fastq.gz | Fastqsanger.gz |
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Project description:Ticks are vectors of different pathogens causing human and animal diseases. Particularly, Rickettsia slovaca is zoonotic infectious bacterium transmitted by Dermacentor ticks, agent of tick-borne lymphadenopathy (TIBOLA), common across Europe. Current studies point to extreme complexity of bacterial induced effects in tick host. Systems biology tools, including proteomics, greatly contribute to understanding of molecular details of pathogen-tick-host interactions. Herein we compared laboratory-infected ticks with uninfected control after four weeks of incubation. Propagation of R. slovaca was confirmed by quantitative PCR. Using DNA was confirmed infection with R. slovaca. By proteomic approach, we discovered 33 differentially abundant gel spots, 23 of them accumulated upon artificial infection with R. slovaca. Modest 6.9% of tick proteome was affected. The protein localizations showing that eight proteins spots might be secreted, three cytoplasmic, two mitochondrial, six likely having multiple localizations, one cell membrane and one nucleus. We identified following proteins defensin, serpins, glycine-rich protein, heat shock protein involved in artificially infected tick vector, Dermacentor reticulatus. Discovered differentially abundant proteins should be further evaluated as targets to block the transmission of bacterial pathogen.
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Project description:There has been an emergence and expansion of tick-borne diseases in Europe, Asia and North America in recent years, including Lyme disease, tick-borne encephalitis, and human anaplasmosis. The primary tick vectors implicated are hard ticks of the Ixodes genera. Although much is known about the host response to these bacterial and viral pathogens, there is limited knowledge of the cellular responses to infection within the tick vector. The bacterium Anaplasma phagocytophilum (A. phagocytophilum), is able to bypass apoptotic processes in ticks, enabling infection to proceed. However, the tick cellular responses to infection with the flaviviruses tick-borne encephalitis virus (TBEV) and louping ill virus (LIV), which cause tick-borne encephalitis and louping ill respectively, are less clear. Infection of an Ixodes ricinus (I. ricinus) tick cell line with the viruses LIV and TBEV, and the bacterium A. phagocytophilum, identified activation of common and distinct cellular pathways. In particular, commonly-upregulated genes included those that modulate apoptotic pathways (HSP70), putative anti-pathogen genes (FKBP and XBL1), and genes that influence the tick innate immune response, including selective activation of toll genes. These data provide an insight into potentially key genes involved in the tick cellular response to viral or bacterial infection.
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Project description:Here, we challenged rabbits with repeated feeding of Ixodes ricinus adults and observed the formation of specific antibodies against several tick salivary proteins. To identify the salivary antigens, isolated immunoglobulins from repeatedly infested rabbits were utilised for a pull-down from the saliva of pilocarpine-treated ticks. Eluted antigens were separated on 1D SDS-PAGE and analysed by peptide mass fingerprinting. To increase the authenticity of immunogens identified, we also performed, for the first time, de novo assembly of the sialome from I. ricinus females fed for six days, a timepoint used for pilocarpine-salivation.
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