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Transcriptome alterations of hiPSC-CMs after alcohol treatment, alcohol and losartan co-treatment

ABSTRACT: Transcriptome alterations of hiPSC-CMs after alcohol treatment, alcohol and losartan co-treatment

PROVIDER: PRJNA802832 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17862805.fastq.gz	Fastqsanger.gz
	SRR17862806.fastq.gz	Fastqsanger.gz
	SRR17862807.fastq.gz	Fastqsanger.gz
	SRR17862808.fastq.gz	Fastqsanger.gz
	SRR17862809.fastq.gz	Fastqsanger.gz

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Transcriptome alterations of hiPSC-CMs after alcohol treatment, alcohol and losartan co-treatment

Project description:In order to investigate the changes of hiPSC-CMS transcriptome after alcohol treatment and whether losartan has an effect on the changes of hiPSC-CM transcriptome after alcohol treatment, RNAseq was performed on hiPSC-CMs treated with 100mM alcohol and 100mM alcohol co-treated with1uM losartan.

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Transcriptome analysis of hiPSC-CMs carrying FLNC mutations

Project description:In this study we performed transcriptome analysis of cardiomyocytes (CMs) derived from two hiPSC lines of patients with V2264M and R1267Q mutations in FLNC gene. hiPSC cell line were obtained from patient’s PBMCs with FLNC mutation using CytoTune-iPS 2.0 Sendai Reprogramming Kit (Thermo Fisher Scientific). Detailed information about iPSC line characterization can be found in hiPSCreg database: (FAMRCi011-A and FAMRCi009). Information about FAMRCi009 is earlier published DOI: 10.1016/j.scr.2021.102640.

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Global gene expression profiles of cardiomyocytes differentiated from human pluripotent stem cells (hiPSC-CMs) in 3D culture exposed to ethanol

Project description:Methods: RNA-seq libraries were prepared using the Illumina TruSeq RNA kit and the TrueSeq method was employed for mRNA enrichment. The libraries were quantified and samples were multiplexed in each lane of the flowcell. Cluster generation was performed and then sequenced on the Illumina HiSeq2500 system. Reads were mapped on the Human Genome Reference and normalized expression table was generated. Results: RNA-seq results reveal gene expression of cardiac toxicity in hiPSC-CMs that are consistent with alcohol-induced pathophysiology observed in animal models. For example MMP9 is among the top 5 upregulated genes in ethanol-treated hiPSC-CMs, MMP9 concentrations are significantly higher in human sera of chronic alcohol abusers and MMP9 mRNA and protein levels are increased in the myocardium of rats following acute ethanol exposure. Conclusions: Data demonstrate significant alteration in gene expression, among the top 60 genes significantly altered by ethanol exposure, 8 genes are involved in ion channels, which may be in part contributing to the abnormal intracellular Ca2+ transients. Ethanol up-regulated the expression of genes associated with collagen metabolism and extracellular matrix (MMP9, EMID1, COL14A1), most of the downregulated genes are involved in cardiovascular system development (NPPB, DNAAF3), actin filament-based process (LMOD2, MYH4) and muscle contraction (MYL2). These findings are consistent with previous studies showing a correlation between alcohol exposure and defects in heart and circulatory system development.

2019-01-31 | GSE125917 | GEO

Transcriptome analysis of hiPSC-CMs carrying FLNC mutations

Project description:Transcriptome analysis of hiPSC-CMs carrying FLNC mutations

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Project description:We performed a genome-wide analysis of m6A mRNA methylation in SUN-treated human pluripotent stem cells induced myocardial cells (hiPSC-CMs) by methylated RNA immunoprecipitation sequencing (MeRIP-seq).

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The effect of doxorubicin treatment on variant rs22297749/S427L)-dependent transcriptome changes in patient-derived human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs).

Project description:We investigate transcriptome changes of variant S427L and control hiPSC–CMs in response to 1 µM doxorubicin for 24 h, using an unbiased approach

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Project description:The goal of this study was to identify differential alternative splicing events in RBPMS2-null hiPSC-CMs.

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Gene expression profile in response to HIF-1α inhibition together with PPARα activation and the postnatal factors (T3, IGF-1 and dexamethasone) in hiPSC-CMs

Project description:Methods: RNA-seq libraries were prepared using the Illumina TruSeq technology. The libraries were quantified and samples were multiplexed in each lane of the flowcell. Cluster generation was performed and then sequenced on the Illumina HiSeq2500 system. Reads were mapped on the Human Genome Reference (GRCh38) and normalized expression table was generated. Results: Among differentially expressed genes, compared with DMSO-treated hiPSC-CMs, 505 genes were upregulated in FM+WY+TID-treated hiPSC-CMs, with 72 genes commonly upregulated in both FM+WY+TID-treated hiPSC-CMs and LV groups and 949 genes were downregulated in FM+WY+TID-treated hiPSC-CMs and 2137 genes were downregulated in LV, with 437 genes downregulated in both FM+WY+TID-treated hiPSC-CMs and LV compared with DMSO-treated hiPSC-CMs . Conclusions: Data demonstrate increased expression of genes associated with many metabolic processes which are also highly enriched in human pediatric heart samples including many interconnected metabolic processes that are upstream of lipid metabolism and FAO, agreeing with the shift to FAO for energy utilization in more mature CMs, and decreased expression of genes involved in developmental processes, adhesion and signaling in both FM+WY+TID-treated hiPSC-CMs and LV. The overlap in both upregulated and downregulated genes in both groups confirmed an advanced degree of cardiomyocyte maturation in response to FM+WY+TID.

2019-01-30 | GSE125862 | GEO

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