Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Viral mimicry of Interleukin-17A by SARS-COV-2 ORF8

ABSTRACT: Viral mimicry of Interleukin-17A by SARS-COV-2 ORF8

PROVIDER: PRJNA804953 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR17965700.fastq.gz	Fastqsanger.gz
	SRR17965701.fastq.gz	Fastqsanger.gz
	SRR17965702.fastq.gz	Fastqsanger.gz
	SRR17965703.fastq.gz	Fastqsanger.gz
	SRR17965704.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 24

Similar Datasets

Viral mimicry of Interleukin-17A by SARS-COV-2 ORF8

Project description:SARS-CoV-2 infection triggers cytokine-mediated inflammation, leading to a myriad of clinical presentations in COVID19. The SARS-CoV-2 ORF8 is a secreted and rapidly evolving glycoprotein. Patients infected with SARS-CoV-2 ORF8-deleted variants are associated with mild disease outcomes, but the molecular mechanism this behind is unknown. Here, we report that SARS-CoV-2 ORF8 is a viral cytokine that is similar but distinct from interleukin 17A (IL-17A) as it induces stronger and broader human IL-17 receptor (hIL-17R) signaling than IL-17A. ORF8 primarily targeted blood monocytes and induced the heterodimerization of hIL-17RA and hIL-17RC, triggering robust inflammatory response. Transcriptome analysis revealed that besides its activation of the hIL-17R pathway, ORF8 upregulated gene expressions for fibrosis signaling and coagulation dysregulation. A naturally occurring ORF8 L84S variant that highly associated with mild COVID-19 showed reduced hIL-17RA binding and attenuated inflammatory responses. This study discovers SARS-CoV-2 ORF8 as a viral mimicry of IL-17 cytokine to contribute COVID-19 severe inflammation.

2022-03-11 | GSE196455 | GEO

ATACseq analysis of HEK cells transfected with SARS-CoV-2 protein Orf8

Project description:Analysis of histone mimicry by SARS-CoV-2 Orf8 encoded protein

2022-07-20 | GSE186623 | GEO

ChIP-seq analysis of HEK cells transfected with SARS-CoV-2 protein Orf8

Project description:Analysis of histone mimicry by SARS-CoV-2 Orf8 encoded protein

2023-12-17 | GSE163676 | GEO

CUT&TAG seq analysis of HEK cells transfected with SARS-CoV-2 protein Orf8

Project description:Analysis of histone mimicry by SARS-CoV-2 Orf8 encoded protein

2022-07-20 | GSE186624 | GEO

RNAseq analysis of HEK293T expressing GFP, Orf8, or Orf8 with an ARKSAP deletion

Project description:Analysis of histone mimicry by SARS-CoV-2 Orf8 encoded protein

2022-07-20 | GSE186625 | GEO

Analysis of histone mimicry by SARS-CoV-2 Orf8 encoded protein

Project description:Analysis of histone mimicry by SARS-CoV-2 Orf8 encoded protein

| PRJNA774809 | ENA

Interleukin-17A based dysregulation of Human Primary Keratinocyte proteome

Project description:To delineate mechanisms for psoriasis pathogenesis driven by the interleukin-17A, proteomic dysregulations were studied in a Human Primary Keratinocyte model system. Label-free quantification was performed and fold-changes were obtained for abundances of proteins in IL-17A treated keratinocytes versus those from IL-17A treated keratinocytes. Briefly, Human Primary Keratinocytes were isolated and treated with the cytokine IL-17A (50ng/ml) in incomplete media devoid of any growth factors. Tryptic digested and desalted peptide samples were injected in Thermoscientific Q-Exactive Plus instruments through EasyNLC HPLC autosampler. The instruments were set to MS1 resolution of 70000 and MS2 resolution of 17500. The acquisition experiments were optimized to run on 120 min gradients. The MS spectra were analyzed using the Thermoscientific mass informatics platform Proteome discoverer version 2.2. The common workflows for discovery proteomics were used with Mascot and SequestHT as search engines. This dataset helped to simulate the IL-17A-driven inflammation in keratinocytes and uncovered many putative druggable targets in the context of psoriasis.

2022-08-11 | MSV000090116 | MassIVE

ATACseq analysis of A549 cells infected with SARS-CoV-2

Project description:Analysis of histone mimicry by SARS-CoV-2 Orf8 encoded protein

2022-07-20 | GSE186621 | GEO

RNAseq analysis of SARS-CoV-2 infection of iAT2 cells

Project description:Analysis of histone mimicry by SARS-CoV-2 Orf8 encoded protein

2022-07-20 | GSE186627 | GEO

RNAseq analysis of SARS-CoV-2 infection of A549ACE cells

Project description:Analysis of histone mimicry by SARS-CoV-2 Orf8 encoded protein

2022-07-20 | GSE186622 | GEO