Ontology highlight
ABSTRACT:
PROVIDER: PRJNA805185 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Major classes of hormone mimics that have been studied include environmental estrogens and androgens, but recent studies have also demonstrated the significant impacts of natural and synthetic progesterones in the environment. The objective of this study was to evaluate the molecular and physiological impacts of progestin, anti-progestin, and mixture exposures in the Eastern Mosquitofish (G. holbrooki). By comparison of gene expression profiles and modulated biological processes in the three groups, it was determined that mifepristone acts more as a progestin than as an anti-progestin, as has also been demonstrated in other species of fish. This work contributes to the overall knowledge of the impacts of this class of chemical contaminats on aquatic organisms, which are a sentinel species for pollutants as aquatic ecosystems often become a reservoir for anthropogenic contaminants.
2014-09-01 | GSE55518 | GEO
Project description:The widespread occurrence of psychoactive pollutants in surface waters raises concerns for aquatic ecosystems, yet impacts beyond behavioral disruption remain poorly understood. Designed to target neurotransmitter (NT) systems in humans, these contaminants may also interfere with conserved NT pathways in fish. Here we provide the first evidence that fish sperm contain NTs and their receptors, indicating an active NT-mediated signaling system essential for sperm function. Using European perch (Perca fluviatilis) exposed to environmentally relevant concentrations of methamphetamine, we quantified NTs in brain, gonads, and sperm, assessed tissue-specific bioaccumulation, and evaluated sperm performance. Methamphetamine accumulated across tissues, modified NT profiles, and altered sperm motility and velocity. These findings reveal a previously unrecognized pathway of reproductive vulnerability, identifying NT signaling in sperm as a novel target of psychoactive pollutants with potential implications for fertilization success and population resilience in aquatic ecosystems.
2025-12-11 | PXD061699 | Pride
Project description:Toxic chemical contaminants have variety of detrimental effects on various species and the impact of pollutants on ecosystems has become an urgent issue. However, very limited species have been examined to date and those studies are mainly limited to vertebrates. In this study, we aimed to establish an ecotoxicogenomic bases for Daphnia magna. Based on a daphnia EST database, we made oligonucleotide-based DNA microarray that has high reproducibility. The DNA microarray was applied to evaluate gene expression profiles of daphnid exposed to chemicals. Characteristic gene expression patterns depending on chemicals indicate that the Daphnia microarray can be used for mechanistic understanding of chemical toxicity. Although acute toxicity test or reproductive toxicity test can provide hazardous concentrations of chemicals, they give no information about mode of action. Our study can be a breakthrough for the evaluation of chemical toxicity on environmental organisms. Keywords: Chemical response
2005-12-31 | GSE3445 | GEO
Project description:Impact of polypropylene microplastics and chemical pollutants on sea bass (Dicentrarchus labrax) gut health
| PRJEB41533 | ENA
Project description:The increase in human population and urbanization are resulting in an increase in the volume of wastewater and urban runoff effluents entering natural ecosystems. These effluents may contain multiple pollutants to which the biological response of aquatic organisms is still poorly understood mainly due to mixture toxicity and interactions with other environmental factors. In this context, RNA sequencing was used to assess the impact of a chronic exposure to wastewater treatment plant and stormwater effluents at the whole-transcriptome level and evaluate the potential physiological outcomes in the Asian clam Corbicula fluminea. We de-novo assembled a transcriptome from C. fluminea digestive gland and identified a set of 3,181 transcripts with altered abundance in response to water quality. The largest differences in transcriptomic profiles were observed between C. fluminea from the reference site and those exposed to wastewater treatment plant effluents. On both anthropogenically impacted sites, most differentially expressed transcripts were involved in signaling pathways in relation to energy metabolism such as mTOR and FoxO, suggesting an energy/nutrient deficit and hypoxic conditions. These conditions were likely responsible for damages to proteins and transcripts in response to wastewater treatment effluents whereas exposure to urban runoff might result in immune and endocrine disruptions. In absence of comprehensive chemical characterization, the RNAseq approach could provide information regarding the mode of action of pollutants and then be useful for the identification of which parameters must be studied at higher integration level in order to diagnose sites where the presence of complex and variable mixtures of chemicals is suspected.
2018-08-28 | GSE104933 | GEO
Project description:CdSe nanoparticles (CdSe NPs) are extensively used in the industry of renewable energies and it is regrettably expectedthat these pollutants will sometime soon appear in marine environmentthrough surface runoff, urban effluents and rivers. Bacteria living in estuarine and coastal sediments will be among the first targets of these new pollutants. The pseudomonads are frequently encountered in these ecosystems. They are involved in several biogeochemical cycles and are known for their high resistance to pollutants. Consequently, this study focussing on the effect of CdSe NPs on the marine strain P. fluorescensBA3SM1 is highly relevant for a number of reasons. First, it aims at improving knowledge about the interactions between bacteria and NPs. This is fundamental to use effectively NPs against pathogenic bacteria. Secondly, this study shows that CdSe NPs of 8 nm in diameter cause a decrease in the secretion of siderophorepyoverdine, a secondary metabolite having a key role in microbial ecology and also employed as a virulence factor in human pathogenic strains such as P. aeruginosa. Consequently, this study highlights that CdSe NPs can have an impact on secondary metabolism of bacteria with environmental and medical implications.
2016-08-17 | PXD004012 | Pride
Project description:A short-term microcosm experiment was conducted to evaluate the impact of wastewater discharge on coastal microbial communities. Coastal seawater was exposed to two types of treated wastewater: (i) unfiltered wastewater, containing nutrients, pollutants, and allochthonous microbes, and (ii) filtered wastewater, which retained only nutrients and pollutants while removing microbial components. Metaproteomic samples were collected from the coastal seawater prior to the experiment and from each experimental flask at the late exponential growth phase to assess microbial functional responses to wastewater exposure.
2025-05-09 | PXD061573 | Pride
Project description:Contaminants of Emerging Concern (CECs) can be measured in waters across the United States, including the tributaries of the Great Lakes. The extent to which these contaminants affect gene expression in aquatic wildlife is unclear. This dataset presents the full hepatic transcriptomes of laboratory reared fathead minnows (Pimephales promelas) caged at multiple sites within the Milwaukee Estuary area of concern and control sites. Following 4 days of in situ exposure, liver tissue was removed from males at each site for RNA extraction and sequencing, yielding a total of 116 samples from which libraries were prepared, pooled, and sequenced. For each exposure site, 179 chemical analytes were also assessed. These data were created with the intention of inviting research on possible transcriptomic changes observed in aquatic species exposed to CECs. Access to both full sequencing reads of animal samples as well as water contaminant data across multiple Great Lakes sites will allow others to explore the health of these ecosystems, in support of the aims of the Great Lakes Restoration Initiative.
2020-01-31 | GSE144301 | GEO
Project description:Major classes of hormone mimics that have been studied include environmental estrogens and androgens, but recent studies have also demonstrated the significant impacts of natural and synthetic progesterones in the environment. The objective of this study was to evaluate the molecular and physiological impacts of progestin, anti-progestin, and mixture exposures in the Eastern Mosquitofish (G. holbrooki). By comparison of gene expression profiles and modulated biological processes in the three groups, it was determined that mifepristone acts more as a progestin than as an anti-progestin, as has also been demonstrated in other species of fish. This work contributes to the overall knowledge of the impacts of this class of chemical contaminats on aquatic organisms, which are a sentinel species for pollutants as aquatic ecosystems often become a reservoir for anthropogenic contaminants. G. holbrooki adult males and females were exposed to one of the following conditions: vehicle control (ethanol), 100 ng/L of levonorgestrel, 100 ng/L of mifepristone, or a mixture of both levonorgestrel and mifepristone. All exposures were conducted for 48 hours with water changed and chemicals renewed daily. Fish were anesthetized using 100 mg/L Benzocaine (Ethyl 4-aminobenzoate). Livers were removed and stored in RNAlater (Qiagen, Hilden, Germany) overnight at 4 C before storage at -80 C. RNA was isolated from the livers using TRIzol (Invitrogen, Grand Island, USA), hydrated using RNAsecure (Ambion, Grand Island, USA), and DNase treated using the Turbo DNA-free kit (Ambion, Grand Island, USA). Four oocyte-development stage-matched RNA samples per treatment were evaluated for RNA integrity using the 2100 BioAnalyzer (Agilent, Santa Clara, USA). The range of RIN values was 8.2-9.6
2014-09-01 | E-GEOD-55518 | biostudies-arrayexpress
Project description:Polyethylene microplastics are pervasive environmental pollutants that pose potential risks to aquatic organisms. This study investigates the effects of polyethylene microplastics on zebrafish (Danio rerio) embryo development, with a focus on whether the chorion, a protective barrier surrounding the embryo, is effective in blocking polyethylene penetration. Contrary to previous findings that suggested the chorion could prevent larger microplastics (>0.7 µm) from entering, our study demonstrates that polyethylene particles sized 1-4 µm can still negatively impact embryo development without dechorionation. Embryos were exposed to polyethylene at concentrations of 0.01, 0.1, and 1 mg/L, followed by RNA sequencing to assess gene expression changes. Despite no significant differences in survival, hatching, or body length between control and treated groups, a significant reduction in heart rate was observed at higher concentrations, indicating potential sub-lethal cardiotoxicity. Further, qRT-PCR validation confirmed significant downregulation of key heart development-related genes, particularly fbln1 and fn1b, in polyethylene-exposed embryos. Additionally, polyethylene exposure also impacted Daphnia magna, reducing heart rate and delaying growth. These findings highlight the ability of polyethylene microplastics to penetrate natural barriers such as the chorion and induce physiological and developmental changes. Our results emphasize the need for further research into the long-term effects of microplastic exposure on aquatic ecosystems.
2025-11-30 | GSE281865 | GEO