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Targeting S6K1 and S6K2 by siRNA and sgRNA in LN229 GBM cells

ABSTRACT: Targeting S6K1 and S6K2 by siRNA and sgRNA in LN229 GBM cells

PROVIDER: PRJNA805545 | ENA |

REPOSITORIES: ENA

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Targeting S6K1 and S6K2 by siRNA and sgRNA in LN229 GBM cells

Project description:To systematically identify the function of S6K1 and S6K2 in glioblastoma cells, LN229 cells were stably transduced with Cas9, control non-targeting sgRNA (sgNT), or sgRNAs targeting S6K1 or S6K2. The cells were then transiently transfected with non-targeting siRNAs (siNT), or siRNA pools targeting S6K1 or S6K2. RNA sequencing was performed to compare cellular responses to inactivation of single or combination S6 kinases.

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