Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ABSTRACT: Oxygen tolerance testing of DGG-B, a methanogenic benzene-degrading enrichment culture

PROVIDER: PRJNA807302 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR18048400_1.fastq.gz	Fastqsanger.gz
	SRR18048400_2.fastq.gz	Fastqsanger.gz
	SRR18048401_1.fastq.gz	Fastqsanger.gz
	SRR18048401_2.fastq.gz	Fastqsanger.gz
	SRR18048402_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 70

Similar Datasets

hydrocarbon metagenome

Project description:Methanogenic benzene-degrading enrichment cultures

| PRJDB10156 | ENA

sediment metagenome

Project description:Metagenomes from methanogenic benzene-degrading culture

| PRJNA802382 | ENA

groundwater metagenome

Project description:Methanogenic benzene-degrading consortia

| PRJNA264900 | ENA

mixed culture metagenome

Project description:Methanogenic naphtha degrading enrichment culture Metagenome

| PRJNA271642 | ENA

mixed culture metagenome

Project description:Methanogenic toluene degrading enrichment culture Metagenome

| PRJNA271623 | ENA

Project description:Electron donor trials testing for enrichment of Ca. Nealsonbacteria from a methanogenic benzene-degrading consortium

| PRJNA830497 | ENA

methanogenic benzene-degrading enrichment culture

Project description:Methanogenic benzene-degrading microbial community

| PRJDB3983 | ENA

bioreactor metagenome

Project description:Benzene-degrading methanogenic consortium metagenomic assembly

| PRJNA281117 | ENA

mixed culture metagenome

Project description:Methanogenic short chain alkane degrading enrichment culture Metagenome

| PRJNA271643 | ENA

Anaerobic oxidation of benzene by Geobacter metallireducens

Project description:Anaerobic activation of benzene is expected to represent a novel biochemistry of environmental significance but research into the mechanisms has been stymied by a lack of a genetically tractable pure culture which unequivocally does not use molecular oxygen to activate benzene. Geobacter metallireducens grew in a medium in which benzene was the sole electron donor and Fe(III) was the sole electron acceptor with a stoichiometry of benzene loss and Fe(III) reduction consistent with benzene oxidation to carbon dioxide coupled with Fe(III) reduction. Phenol labeled with 18O was produced when the medium was labeled with H218O, as expected for a true anaerobic conversion of benzene to phenol. Gene expression patterns indicated that benzene was metabolized through a phenol intermediate rather than benzoate or toluene. Deletion of ppcB, which encodes a subunit of the phenylphosphate carboxylase, an enzyme required for phenol metabolism, inhibited metabolism of benzene. Deleting genes specific for benzoate or toluene metabolism did not. Comparison of gene expression patterns in cells grown on benzene versus cells grown on phenol revealed genes specifically expressed in benzene-grown cells. Deletion of one of these, Gmet_3376, inhibited anaerobic benzene oxidation, but not the metabolism of phenol, benzoate, or toluene. The availability of a genetically tractable pure culture that can anaerobically convert benzene to phenol with oxygen derived from water should significantly accelerate elucidation of the mechanisms by which benzene can be activated in the absence of molecular oxygen.

2013-04-30 | GSE33794 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data