Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR18078748_1.fastq.gz | Fastqsanger.gz | |||
| SRR18078748_2.fastq.gz | Fastqsanger.gz | |||
| SRR18078749_1.fastq.gz | Fastqsanger.gz | |||
| SRR18078749_2.fastq.gz | Fastqsanger.gz | |||
| SRR18078750_1.fastq.gz | Fastqsanger.gz |
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Similar Datasets
Project description:embryogenesis col0-somatic embryogenesis
| PRJNA143393 | ENA
Project description:Arabidopsis thaliana somatic embryogenesis
| PRJNA422138 | ENA
Project description:Reprogramming in protoplast cultures is a key step for acquiring embryogenic competence by dedifferentiated cells, thus leading to plant regeneration. Therefore, to better understand factors trigging the processes, this work focuses on analysing proteomes, cell wall composition and changes in the expression profile of selected genes. The results demonstrate a significant accumulation of somatic embryogenesis related proteins like late embryogenesis abundant proteins (embryogenic protein-DC-8-like, seed biotin-containing protein) and endochitinases during the developmental path of protoplast cultures supporting somatic embryogenesis events. Additionally, there is an increase in seed storage proteins like vicilin, oleosins, and seed biotin-containing proteins in the cultures. Investigation of somatic embryogenesis-associated transcription factors revealed intensive up-regulation of LEC1 for 50th day of F. tataricum protoplast cultures. Furthermore, we demonstrated the variable distribution of cell wall components like pectin side chains, arabinogalactan proteins (AGP) and extansin (EXT), indicating the acquisition of competence and cellular differentiation.
| MSV000095861 | MassIVE
Project description:Somatic embryogenesis is an important biotechnological technique for large-scale propagation of elite genotypes. Correlations of stage-specific compounds associated with somatic embryo development can help elucidate the ontogenesis of Carica papaya L. somatic embryos and improve tissue culture protocols. To identify the stage-specific proteins that are present during the differentiation of C. papaya somatic embryos, proteomic analyses of embryos at the globular, heart, torpedo and cotyledonary stages were performed. Comparative proteomic analysis revealed a total of 801 proteins, 392 of which were classified as differentially accumulated proteins in at least one of the developmental stages. The globular stage presented a higher number of unique proteins (16), 7 of which were isoforms of 60S ribosomal proteins, suggesting high translational activity at the beginning of somatic embryogenesis. Proteins related to mitochondrial metabolism accumulated to a high degree at the early developmental stages, after which they decreased with increasing development, contributing to cell homeostasis in early somatic embryos. On the other hand, a progressive increase in the accumulation of vicilin, late embryogenesis abundant proteins and chloroplastic proteins leading to somatic embryo maturation was observed. Additionally, the differential accumulation of acetylornithine deacetylase and S-adenosylmethionine synthase 2 proteins correlated with increased contents of putrescine and spermidine, suggesting that polyamine metabolism is important to somatic embryo development. Taken together, the results showed that somatic embryo development in C. papaya is regulated by the differential accumulation of proteins, with ribosomal and mitochondrial proteins being more abundant during the early somatic embryo stages, while proteins involved in seed maturation are more abundant during the late stages.
2020-11-12 | PXD021107 | Pride
Project description:Somatic embryogenesis closely resembles zygotic embryogenesis and hence, it is considered as a model system to explore dynamic events of embryogenesis, at a molecular level. We sequenced three district developmental time points of somatic embryo development in Arabidopsis thaliana with the aim of exploring transcriptomes at a global scale.
2016-01-31 | E-MTAB-2403 | biostudies-arrayexpress