Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

A single-cell atlas of the tumor, stromal and immune microenvironments in pancreatic cancer liver metastasis

ABSTRACT: A single-cell atlas of the tumor, stromal and immune microenvironments in pancreatic cancer liver metastasis

PROVIDER: PRJNA809328 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

A single-cell atlas of the tumor, stromal and immune microenvironments in pancreatic cancer liver metastasis

Project description:Pancreatic ductal adenocarcinoma (PDAC) remains one of the most aggressive and lethal malignant tumors all over the world, with a 5-year survival rate of less than 10%1,2. Usually diagnosed at an advanced stage, PDAC is highly resistant to current therapies, partly due to an extremely high rate of distant metastasis (>80%) at first diagnosis. The liver is the most frequent site of distant metastases for PDAC. Current therapeutic options for PDAC patients with liver metastasis are extremely limited3. Thus, understanding the molecular mechanisms underlying hepatic metastasis of PDAC is urgently needed to help us develop more effective treatments and improve survival for these patients with advanced disease.

2023-04-20 | GSE197177 | GEO

Spatial mapping of thymic stromal microenvironments reveals unique features influencing T lymphoid differentiation

Project description:Interaction of hematopoietic progenitors with the thymic stromal microenvironment induces them to proliferate, adopt the T cell fate, and asymmetrically diverge into multiple T lineages. Progenitors at various developmental stages are stratified among different regions of the thymus, implying that the corresponding microenvironments differ from one another, and provide unique sets of signals to progenitors migrating between them. The nature of these differences remains undefined. Here we use novel physical and computational approaches to characterize these stromal subregions, distinguishing gene expression in microdissected tissues from that of their lymphoid constituents. Using this approach, we comprehensively map gene expression in functionally distinct stromal microenvironments, and identify clusters of genes that define each region. Quite unexpectedly, we find that the central cortex lacks distinctive features of its own, and instead appears to function by sequestering unique microenvironments found at the cortical extremities, and modulating the relative proximity of progenitors moving between them. 4 to 6 weeks old male C57bl6/J were used for microdissection of 3 thymic cortical subregions and thymic medulla or for sorting cortical and medullary thymocytes. These samples were used for subsequent RNA purification, labeling and hybridization to Affymetrix arrays

2009-11-23 | E-GEOD-18281 | biostudies-arrayexpress

Melanoma Brain Metastasis Atlas

Project description:Melanoma Brain Metastasis Atlas

| PRJNA768859 | ENA

Targeting heterogeneous tumor microenvironments in pancreatic cancer mouse models of metastasis by TGFB depletion

Project description:Targeting heterogeneous tumor microenvironments in pancreatic cancer mouse models of metastasis by TGFB depletion

| PRJNA1151736 | ENA

Melanoma Brain Metastasis Atlas [SlideSeq]

Project description:Melanoma Brain Metastasis Atlas [SlideSeq]

| PRJNA823928 | ENA

Spatial mapping of thymic stromal microenvironments reveals unique features influencing T lymphoid differentiation

2009-11-24 | GSE18281 | GEO

Spatially confined sub-tumor microenvironments orchestrate pancreatic cancer pathobiology

Project description:Pancreatic ductal adenocarcinoma (PDAC) remains resistant to most treatments and demonstrates a complex pathobiology. Here, we deconvolute regional heterogeneity in the human PDAC tumor microenvironment (TME), a long-standing obstacle, to define precise stromal contributions to PDAC progression. Large scale integration of histology-guided multiOMICs profiling with clinical data sets and functional in vitro models uncovered two microenvironmental programs in PDAC that were anchored in fibroblast differentiation states. These sub-tumor microenvironments (subTMEs) co-occurred intratumorally and were spatially confined, producing patient-specific cellular and molecular heterogeneity associated with shortened patient survival. Each subTME was uniquely structured to support discrete aspects of tumor biology: reactive regions rich in activated fibroblast communities were immune-hot and promoted aggressive tumor progression while deserted regions enriched in extracellular matrix supported tumor differentiation yet were markedly chemoprotective. In conclusion, PDAC regional heterogeneity derives from biologically distinct reactive and protective TME elements with a defined, active role in PDAC progression.

2021-08-23 | GSE166571 | GEO

Melanoma Brain Metastasis Atlas [TCR-Seq]

Project description:Melanoma Brain Metastasis Atlas [TCR-Seq]

| PRJNA823686 | ENA

Melanoma Brain Metastasis Atlas [patient_cell_lines_ATAC-seq]

Project description:Melanoma Brain Metastasis Atlas [patient_cell_lines_ATAC-seq]

| PRJNA843232 | ENA

Melanoma Brain Metastasis Atlas [patient_cell_lines_RNA-seq]

Project description:Melanoma Brain Metastasis Atlas [patient_cell_lines_RNA-seq]

| PRJNA823684 | ENA