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ABSTRACT: WT-S89G-DMP1 mice

PROVIDER: PRJNA810570 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR18163243_1.fastq.gz	Fastqsanger.gz
	SRR18163243_2.fastq.gz	Fastqsanger.gz
	SRR18163244_1.fastq.gz	Fastqsanger.gz
	SRR18163244_2.fastq.gz	Fastqsanger.gz
	SRR18163245_1.fastq.gz	Fastqsanger.gz

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Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and Nrl-/- Retinal Transcriptomes

Project description:Purpose: To identify the role of glycosylation of dentin matrix protein1 (DMP1), S89G-DMP1 point mutation mouse model was created with changing S89 to glycine(S89G). RNA sequencing were performed to compare the transcriptome differences between the neural stem cells or astrocytes separated form S89G-DMP1 and WT mice. Methods: mRNA profiles of astrocytes and neural stem cell separated from S89G-DMP1 and WT were generated by RNA sequencing. The sequence reads that passed quality filters were analyzed at the transcript isoform level with TopHat2(for astrocytes) or with HISAT2o (for neural stem cells). Results: Differential expression analyses with DESeq2 use raw read counts: 762 transcripts were downregulated and 991 transcripts were up-regulated in S89G-DMP1 astrocytes; 475 transcripts were downregulated and 343 transcripts were up-regulated in S89G-DMP1 neural stem cells. All the differential expressed genes were used for Heatmap analysis and KEGG or GO enrichment analyses. Conclusions: Our study represents the detailed transcriptome changes of astrocytes and neural stem cells with deglycosylated DMP1.

2017-06-08 | GSE99784 | GEO

Single-cell RNA-sequencing of intratibial LLC cancer model in DMP1-Cre Mfn2-/- mice, DMP1-Cre Rhot1-/- mice, and wild-type mice.

Project description:We injected LLC1 cells into the tibia of DMP1-Cre Mfn2-/- mice, DMP1-Cre Rhot1-/- mice, and wild-type mice to study the changes in the cellular composition of the cancer microenvironment after knockout of Mfn2 or Rhot1 in osteocytes.

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Expression data from Dmp1-Cre targeted and non-targeted Cxcl12-abundant reticular (CAR) cells in mouse bone marrow

Project description:In mouse bone marrow, mesenchymal stem cells (MSC) has the potential to form osteocytes, adipocytes and cartilage. In the process of osteogenesis, MSCs differenetiate into stromal cells, such as CAR cells. Osteoblast is responsible for the formation of osteocytes and osteoblasts may be differentiated from a subset of CAR cells. Dmp1-Cre targeted CAR cells are thought to enrich for a osteoblast progenitor population. We used microarrays to detail the gene expression profiles among Dmp1-Cre targeted and non-targeted CAR cells. Gene expression diffferences were compared to support the hypothesis that Dmp1-Cre targeted CAR cells may be enriched for osteoblast progenitors. Dmp1-Cre targeted and non-targeted CAR cells were FACS sorted from three mice. RNA were extracted from these sorted cells and processed for microarray using Affymetrix mogene 1.0 ST chip. Cells from one mouse represent one sample

2016-05-14 | E-GEOD-81399 | biostudies-arrayexpress

Expression data from Dmp1-GFP sorted osteocytes

Project description:Estrogens are well known steroid hormones necessary to maintain bone health. In addition, mechanical loading, which estrogen signaling may intersect with the Wnt/Î²-catenin pathway, is also essential for bone health. As osteocytes are known as the major mechanosensory cells embedded in mineralized bone matrix, osteocyte ERÎ± deletion mice (ERÎ±ÎOcy/ÎOcy) were generated by mating ERÎ± floxed mice with Dmp1-Cre mice to determine functions of ERÎ± in osteocytes. Trabecular bone mineral density of female, but not male ERÎ±ÎOcy/ÎOcy mice was significantly decreased. Bone formation parameters in ERÎ±ÎOcy/ÎOcy were significantly decreased while osteoclast parameters were unchanged. This suggests that ERÎ± in osteocytes exerts osteoprotective function by positively controlling bone formation. To identify potential targets of ERÎ±, gene array analysis of Dmp1-GFP osteocytes FACS sorted from ERÎ±ÎOcy/ÎOcy and control mice was performed. Expression of Mdk and Sostdc1, both known inhibitors of Wnt, were significantly increased without alteration of the mature osteocyte marker Sost or Î²-catenin. Hindlimb unloading exacerbated the trabecular bone loss, but surprisingly cortical bone was resistant. These studies show that ERÎ± in osteocytes has osteoprotective effects in trabecular bone through regulating expression of Wnt antagonists, but conversely plays a negative role in cortical bone loss due to unloading. Wild type and osteocyte-specific Estrogen Receptor alpha knock-out mice were generated. The number of both genotypes of mice was three. Calvarial osteocytes of both genotypes harboring Dmp1-GFP were extracted by sequential enzymatic digestion, followed by FACS Aria sorting and total RNAs were purified for Affymetix GeneChip microarray analysis without pooling.

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Project description:Purpose of arrays were to determine what the effect of deletion of Mbtps1 gene was on gene expression of osteocytes in bone in vivo. DMP1 cre driver was used to delete the Mbtps1 gene in osteocytes and osteoblasts in bone. We then isolated osteocyte enriched bone particles from 40 week old male mice to determine the effect of this deletion on gene expression. We have previously shown that Mbtps1 is needed for transcription of Phex, DMP1, and MEPE genes in osteoblasts in culture. Arrays showed these genes were reduced as expected in osteocytes in vivo. Controls represent osteocyte enriched bone from 40 week old littermates. Also, as expected, Mbtps1 expression was reduced in these knockout mice

2015-12-29 | GSE69975 | GEO

Single-cell RNA-sequencing of osteocyte-derived mitochondria recipient cells in the intra-tibial injection of LLC1 cells murine model.

Project description:LLC1 cells were injected into the tibia of DMP1-Cre PhAM mice to study the intercellular mitochondrial transfer in the bone microenvironment. We used single-cell RNA sequencing (scRNA-seq) to analyze the cellular heterogeneity of osteocyte-derived mitochondria recipient cells.

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Expression data from Dmp1-Cre targeted and non-targeted Cxcl12-abundant reticular (CAR) cells in mouse bone marrow

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Microarray on mouse soleus muscles from DMP1 Cre Mbtps1 knockout mice compared with littermate controls

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