Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ABSTRACT: Rice promoter editing

PROVIDER: PRJNA810966 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR18163209_1.fastq.gz	Fastqsanger.gz
	SRR18163209_2.fastq.gz	Fastqsanger.gz
	SRR18163210_1.fastq.gz	Fastqsanger.gz
	SRR18163210_2.fastq.gz	Fastqsanger.gz
	SRR18163211_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 36

Similar Datasets

Project description:Editing HSPCs on HBG promoter

| PRJNA558438 | ENA

TMT Labeling proteomic analysis of wild and mutant of OsGSTU34 transporter in black rice caryopsis

Project description:TMT labeling proteomic analysis was performed on black rice caryopsis after editing an important anthocyanin transporter gene called OsGSTU34

2023-08-15 | PXD044599 |

Small RNA profiles of the rice non-pollen male sterile line Wuxiang S reveal miRNAs involved in the fertility transition

Project description:Illumina sequencing was employed to examine the expression profiles of rice anther miRNAs from the a non-pollen male sterile line Wuxiang S (WXS), one of photo-thermo sensitive genical male sterile (PTGMS) line rice, during in the fertility transition stage. A total of 493 known miRNAs and 273 novel miRNAs were identified during rice anther development. Based on the number of sequencing reads, a total of 26 miRNAs were discovered to be significant difference expression between WXS(S, Sterility) and WXS(F, Fertility), and the results were partially validated by qRT-PCR. Among these, 11 miRNAs were decreased and 15 miRNAs were increased in WXS(S) compared with WXS(F). The expression patterns for targets of osa-miR156a-j, osa-miR3879, osa-miR159c/d/e, osa-miR171a/c/e/i, osa-miR398b, osa-miR164d, osa-miR528 and osa-miR408 were selectively examined, and the results showed that there was a negative correlation on the expression patterns between miRNAs and their targets. These targets have previously been reported to be related with pollen development and male sterility, suggesting that miRNAs might act as regulators of rice anthers. Furthermore, miRNA editing events were observed. The U-to-C and U-to-A editing phenomenon was validated by molecular cloning and sequencing.

2016-02-14 | GSE74003 | GEO

Small RNA profiles of the rice non-pollen male sterile line Wuxiang S reveal miRNAs involved in the fertility transition

2016-02-14 | E-GEOD-74003 | biostudies-arrayexpress

Project description:FrCas9 genome editing in rice

| PRJNA1050878 | ENA

Project description:FrCas9 genome editing in rice

| PRJNA1054222 | ENA

MiSeq paired-end sequencing of HEK293T cells stably expressing a Cas9/gRNA-dependent editable reporter vector to test novel nucleobase-editing techniques

Project description:The goal of these experiments were to test the on-target and target-adjacent editing efficiencies of different single-nucleobase editing systems. Previous studies have shown that tethering DNA mutating enzymes to Cas9-nickase-UGI complexes results in editing of chromosomal DNA. However, these editing events encompass undesirable target-adjacent nucleobase edits. Here, we characterize a novel approach that reduces the frequency of target-adjacent editing while maintaining a high level of on-target editing.

2020-02-23 | E-MTAB-8742 | biostudies-arrayexpress

The transcriptome of anthers of rice gamyb mutant expressing various GAMYB homologs was measured at the microspore stage.

Project description:To confirm the function of the GAMYB homologs for Selaginella moellendorffii and Physcomitrella patens in rice cells, we examined the expression profile in the anthers of transgenic plants, which OsGAMYB, SmGAMYB, or PpGAMYB2 is expressed under the control of the rice GAMYB promoter in rice gamy mutant.

2011-11-07 | GSE32652 | GEO

Specific combinatorial mutations in MIR396 genes enlarge the grain size and modulate the plant architecture in rice

Project description:miR396 is a key growth regulator in plants, however, the molecular mechanisms underlying its functions remained to be revealed. Here, through systematically gene-editing, we found that among the MIR396 family genes, MIR396e and -f were the main regulators of rice growth. mir396ef mutations could increase the grain yield through significantly enlarging the grain size. In addition, mir396ef mutations promoted the seedling growth and modulated the plant architecture by promoting the elongations of leaves (including leaf blades and sheaths) and panicles but suppressing the elongation of internodes, especially the uppermost internode. Our research reveals that mir396ef mutations promote the growth and organ elongation by significantly increasing the level of the gibberellin (GA) precursor, mevalonic acid (MVA), which subsequently activates the GA pathway. Our results also indicate that miR396 regulates the internode elongation through a different mechanism (probably through controlling SD37 expression) from the GA pathway. These results reveal two pathways by which miR396 regulates rice growth and provide valuable gene-editing targets to increase rice productivity.

2019-07-24 | GSE113319 | GEO

Data-independent acquisition (DIA)-MS analysis for identification of Magnaporthe oryzae secreted protein induces cell death and triggers defense responses in rice

Project description:The rice blast disease, caused by Magnaporthe oryzae , devastates cultivated rice (Oryza sativa L.), resulting in extensive global crop loss. We employed a label-free quantitative proteomics approach to discover novel proteins associated with M. oryzae pathogenicity and rice defense. We identified 990 significantly modulated proteins in rice leaves including various pattern recognition receptors (PRRs) and pathogenesis-related (PR) proteins that were induced in response to M. oryzae inoculation. Additionally, 123 M. oryzae proteins were also identified and screened for their cell death-inducing activity by an in-silico approach. Among these, we found a novel protein MoXYL1 (endo-1,4-beta-xylanase) protein, which induces cell death in Nicotiana benthamiana leaves. Transgenic rice plants (PDUF26::MoXYL1) expressing MoXYL1 derived by rice domain of unknown function protein 26 (DUF26) promoter exhibited resistance against the M. oryzae and Cochliobolus miyabeanus and enhanced expression of pathogen-responsive genes and hormone-related genes. Furthermore, the application of data-independent acquisition (DIA) mass spectrometry (MS)-based proteomics on these transgenic rice plants revealed 1,833 significantly modulated proteins in response to M. oryzae, with 219 and 410 proteins responsive to MoXYL1 and M. oryzae, respectively. Based on these results, we propose a signaling network model induced by MoXYL1 and M. oryzae. In summary, our findings highlight the crucial role of MoXYL1 in rice innate immunity against M. oryzae and its potential to enhance rice disease resistance.

2024-03-28 | PXD045543 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data