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Exposure of PBMCs to SARS-CoV-2, as opposed to SARS-CoV, induces cell-intrinsic type I innate immunity responses in several immune cell types in the absence of productive infection

ABSTRACT: Exposure of PBMCs to SARS-CoV-2, as opposed to SARS-CoV, induces cell-intrinsic type I innate immunity responses in several immune cell types in the absence of productive infection

PROVIDER: PRJNA811519 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR18183346_1.fastq.gz	Fastqsanger.gz
	SRR18183346_2.fastq.gz	Fastqsanger.gz
	SRR18183347_1.fastq.gz	Fastqsanger.gz
	SRR18183347_2.fastq.gz	Fastqsanger.gz
	SRR18183348_1.fastq.gz	Fastqsanger.gz

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Exposure of PBMCs to SARS-CoV-2, as opposed to SARS-CoV, induces cell-intrinsic type I innate immunity responses in several immune cell types in the absence of productive infection

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Conjunctival epithelial cells resist productive SARS-CoV-2 infection

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SHAE004: SARS-CoV, SARS-dORF6 and SARS-BatSRBD infection of HAE cultures.

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Project description:For the assessment of host response dynamics to SARS-CoV and SARS-CoV-2 infections in human airway epithelial cells at ambient temperature corresponding to the upper or lower respiratory tract. We performed a temporal transcriptome analysis on human airway epithelial cell (hAEC) cultures infected with SARS-CoV and SARS-CoV-2, as well as uninfected hAEC cultures, incubated either at 33°C or 37°C. hAEC cultures were harvested at 24, 48 72, 96 hpi and processed for Bulk RNA Barcoding and sequencing (BRB-seq), which allows a rapid and sensitive genome-wide transcriptomic analysis in a highly multiplexed manner. Transcriptome data was obtained from a total of 7 biological donors for pairwise comparisons of SARS-CoV or SARS-CoV-2 virus-infected to unexposed hAEC cultures at respective time points and temperatures.

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Conjunctival epithelial cells resist productive SARS-CoV-2 infection

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SHAE003: SARS-CoV, SARS-dORF6 and SARS-BatSRBD infection of HAE cultures.

Project description:HAE cultures were infected with SARS-CoV, SARS-ddORF6 or SARS-BatSRBD and were directly compared to A/CA/04/2009 H1N1 influenza-infected cultures. Cell samples were collected at various hours post-infection for analysis. Time Points = 0, 12, 24, 36, 48, 60, 72, 84 and 96 hrs post-infection for SARS-CoV. Time Points = 0, 24, 48, 60, 72, 84 and 96 hrs post-infection forSARS-ddORF6 and SARS-BatSRBD. Time Points = 0, 6, 12, 18, 24, 36 and 48 hrs post-infection for H1N1. Done in triplicate/quadruplicate for RNA Triplicates/quadruplicates are defined as 3/4 different wells, plated at the same time and using the same cell stock for all replicates. Time matched mocks done in triplicate from same cell stock as rest of samples. Culture medium (the same as what the virus stock is in) will be used for the mock infections. Infection was done at an MOI of 2.

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