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Transcriptome profiling for seed abortion in Ponkan (Citrus reticulata)

ABSTRACT: Transcriptome profiling for seed abortion in Ponkan (Citrus reticulata)

PROVIDER: PRJNA811620 | ENA |

REPOSITORIES: ENA

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Transcriptome profiling for seed abortion in Ponkan (Citrus reticulata)

Project description:Seed developmental arrest is one of the early phenotypes of seed abortion. However, the molecular mechanism underlying seed developmental arrest of citrus is still unclear. In this study, laser capture microdissection (LCM) was used to accurately divide the seeds of seedless Ponkan ‘Huagan No.4’ (Citrus reticulata) (HG) and seeded Ponkan ‘Egan No.1’ (Citrus reticulata) (EG) into nucellus and integument/seed coat tissues. The captured tissues were used for subsequent RNA-seq. Moreover, single-molecule real-time (SMRT) sequencing was used to generate full-length transcripts of EG, which were used as reference transcripts for RNA-seq. These data can be utilized to analyse the causes of citrus seedlessness formation and the molecular regulatory network in the process of seed abortion.

2023-05-08 | GSE197716 | GEO

Transcriptome analysis of Citrus reticulata ovule early development based on laser capture microdissection

Project description:Ovule developmental arrest is one of the early phenotypes of seed abortion. However, the molecular mechanism underlying ovule developmental arrest of citrus is still unclear. In this study, laser capture microdissection (LCM) was used to accurately divide the ovules of seedless Ponkan ‘Huagan NO.4’ (Citrus reticulata) (MT) and seeded Ponkan ‘Egan NO.1’ (Citrus reticulata) (WT) into nucellus and integument tissues. The captured tissues were used for subsequent RNA-seq. Moreover, single-molecule real-time (SMRT) sequencing was used to generate full-length transcripts of WT, which were used as reference transcripts for RNA-seq. These data can be utilized to analyse the causes of citrus seedlessness formation and the molecular regulatory network in the process of ovule abortion.

2024-09-01 | GSE183639 | GEO

Expression data from postharvest Ponkan (Citrus reticulata Blanco) fruit under cold storage

Project description:Low temperature storage of citrus fruits are susceptible to chilling disorder symptoms that impact fruit quality. Understanding the molecular frame underlying the cold storage process will provide a basic guidance for practical control. We used Affymetrix Citrus GeneChip to examine the transcriptional changes in cold-stored Citrus reticulata Blanco cv. ‘Ponkan’ pulp tissue for three successive months.

2009-12-18 | GSE19543 | GEO

Laser capture microdissection-based spatiotemporal transcriptomes uncover regulatory networks during seed abortion in Ponkan (Citrus reticulate)

Project description:Seed abortion is one of the important reasons for the formation of seedless characters in citrus. However, the molecular mechanism of seed abortion in citrus is still unclear. We identified several genes that may play an important role in seed abortion of 'Huagan 4', such as CrWRKY74. The downstream regulatory network of CrWRKY74 was identified based on DAP seq analysis to gain insight into the molecular mechanism related to seed abortion.

2023-05-08 | GSE215889 | GEO

Expression data from postharvest Ponkan (Citrus reticulata Blanco) fruit under cold storage

Project description:Low temperature storage of citrus fruits are susceptible to chilling disorder symptoms that impact fruit quality. Understanding the molecular frame underlying the cold storage process will provide a basic guidance for practical control. We used Affymetrix Citrus GeneChip to examine the transcriptional changes in cold-stored Citrus reticulata Blanco cv. âPonkanâ pulp tissue for three successive months. Ponkan postharvest fruits were under cold storage for three successive months. The pulp tissue was used for RNA extraction and hybridization on Affymetrix Citrus Genome microarrays.

2010-05-17 | E-GEOD-19543 | biostudies-arrayexpress

Citrus reticulata cultivar:Ponkan

Project description:Citrus reticulata cultivar:Ponkan Transcriptome or Gene expression

| PRJNA758487 | ENA

Citrus reticulata cultivar:Ponkan

Project description:Citrus reticulata cultivar:Ponkan Transcriptome or Gene expression

| PRJNA318724 | ENA

Citrus reticulata strain:Ponkan mandarin

Project description:Citrus reticulata strain:Ponkan mandarin Genome sequencing

| PRJNA225963 | ENA

Citrus reticulata strain:Ponkan mandarin

Project description:Citrus reticulata strain:Ponkan mandarin Transcriptome or Gene expression

| PRJNA157945 | ENA

Laser capture microdissection-based spatiotemporal transcriptomes uncover regulatory networks during seed abortion in Ponkan (Citrus reticulate)

Project description:Laser capture microdissection-based spatiotemporal transcriptomes uncover regulatory networks during seed abortion in Ponkan (Citrus reticulate)

| PRJNA891233 | ENA

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