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Laser capture microdissection-based spatiotemporal transcriptomes uncover regulatory networks during seed abortion in Ponkan (Citrus reticulate)

ABSTRACT: Laser capture microdissection-based spatiotemporal transcriptomes uncover regulatory networks during seed abortion in Ponkan (Citrus reticulate)

PROVIDER: PRJNA891233 | ENA |

REPOSITORIES: ENA

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Laser capture microdissection-based spatiotemporal transcriptomes uncover regulatory networks during seed abortion in Ponkan (Citrus reticulate)

Project description:Seed abortion is one of the important reasons for the formation of seedless characters in citrus. However, the molecular mechanism of seed abortion in citrus is still unclear. We identified several genes that may play an important role in seed abortion of 'Huagan 4', such as CrWRKY74. The downstream regulatory network of CrWRKY74 was identified based on DAP seq analysis to gain insight into the molecular mechanism related to seed abortion.

2023-05-08 | GSE215889 | GEO

Transcriptome profiling for seed abortion in Ponkan (Citrus reticulata)

Project description:Seed developmental arrest is one of the early phenotypes of seed abortion. However, the molecular mechanism underlying seed developmental arrest of citrus is still unclear. In this study, laser capture microdissection (LCM) was used to accurately divide the seeds of seedless Ponkan ‘Huagan No.4’ (Citrus reticulata) (HG) and seeded Ponkan ‘Egan No.1’ (Citrus reticulata) (EG) into nucellus and integument/seed coat tissues. The captured tissues were used for subsequent RNA-seq. Moreover, single-molecule real-time (SMRT) sequencing was used to generate full-length transcripts of EG, which were used as reference transcripts for RNA-seq. These data can be utilized to analyse the causes of citrus seedlessness formation and the molecular regulatory network in the process of seed abortion.

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Transcriptome analysis of Citrus reticulata ovule early development based on laser capture microdissection

Project description:Ovule developmental arrest is one of the early phenotypes of seed abortion. However, the molecular mechanism underlying ovule developmental arrest of citrus is still unclear. In this study, laser capture microdissection (LCM) was used to accurately divide the ovules of seedless Ponkan ‘Huagan NO.4’ (Citrus reticulata) (MT) and seeded Ponkan ‘Egan NO.1’ (Citrus reticulata) (WT) into nucellus and integument tissues. The captured tissues were used for subsequent RNA-seq. Moreover, single-molecule real-time (SMRT) sequencing was used to generate full-length transcripts of WT, which were used as reference transcripts for RNA-seq. These data can be utilized to analyse the causes of citrus seedlessness formation and the molecular regulatory network in the process of ovule abortion.

2024-09-01 | GSE183639 | GEO

Transcriptome profiling for seed abortion in Ponkan (Citrus reticulata)

Project description:Transcriptome profiling for seed abortion in Ponkan (Citrus reticulata)

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Mouse placenta in spontaneous abortion

Project description:Genetic profiles of mouse placenta were examined especially at the implantation sites of causing spontaneous abortion at day 14.5 of pregnancy. There is a number of the suffered patients from the recurrent spontaneous abortion by unknown causes. It is an important process to understand genetic profiles in the abortion placentas for the investigation of specific causes to induce rejection of normal embryos from the maternal attack. Our study was carried out on the laboratory mice targeted spontaneous abortion to approximate human disease models.

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Citrus hybrid cultivar cultivar:Ponkan mandarin

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Project description:gnp07_regeneome_microdissectionbias - microdissection vs no microdissection - Transcriptome bias induce by protocols used in microdissection - To compare RNA from flowers and RNA from microdissected flowers to know bias induce by microdissection (dissection from all tissues were collected to have a representation of entire flower)

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Laser Capture Microdissection and RNA Sequencing Human Adamantinomatous Craniopharyngioma

Project description:Laser capture microdissection of cluster cells, palisading epithelium and glial tissue from 2 cases of human adamantinomatous craniopharyngioma. 1 case performed in duplicate.

2018-04-11 | E-MTAB-5266 | biostudies-arrayexpress

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