Project description:Tumor tissues contain various cell types and we focus on the stromal cell component in the tumor microenvironment to study their role and interaction. We isolated perivascular cells from two different fibrosarcoma tumor types and compared gene expression patterns.
Project description:Inflammatory crosstalk between perivascular adipose tissue and and blood vessel wall may contribute to atherosclerosis pathogenesis, and exhibits more pro-inflammatory than adipogenic phenotype than subcutaneous adipocytes. To identify a genomic basis for biologic differences, we performed genome-wide expression to identiy expression genes differentially regulated between perivascular and subcutaneous adipocytes.for biologic differences. We performed global gene expression analyses on in vitro differentiated adipocytes from human left coronary artery perivascular adipose tissue and subcutaneous adipose tissues derived from unrelated donors who were non-obese and did not have any known metabolic or atherosclerotic disease.
Project description:Aim of this experiment was to characterize whether SPP1 released from the hippocampal perivascular space could imprint microglia transcriptional states. We performed scRNA-seq analysis on sorted CD140a+ perivascular fibroblast (PVF), CD45highCD11intCD206+ perivascular macrophage (PVM) and CD45intCD11intCX3CR1high microglial cell from dissected hippocampi of 6-month old wild type vs SPP1KO/KO vs AppNL-F mice vs AppNL-FxSpp1KO/KO mice. Cells were isolated from dissected hippocampi as previously described (Sala Frigerio et al.,2019).
Project description:Phosphoproteome analyses of colorectal cancer tissues from 20 patients (20 tumors and 20 tumor-adjacent normal tissues) were carried out using IMAC-based posphopeptide enrichment followed by SCX-RP-MS/MS. The obtained results were searched against UniProt DB by Mascot, and the identified peptides were filtered at 1% FDR thresholds by searching against the randomized decoy DB at peptide level. This dataset with the PX complete submission format will be further used to generate a customized DB in this jPOST project.
Project description:Inflammatory crosstalk between perivascular adipose tissue and and blood vessel wall may contribute to atherosclerosis pathogenesis, and exhibits more pro-inflammatory than adipogenic phenotype than subcutaneous adipocytes. To identify a genomic basis for biologic differences, we performed genome-wide expression to identiy expression genes differentially regulated between perivascular and subcutaneous adipocytes.for biologic differences.
Project description:We performed microarray expression profiling to analyze the differentially expressed genes between 19 human glioma tissues and corresponding adjacent non-tumor tissues.