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Transcriptomic analysis of the effect of cytoplasmic Rat1 (cRat1)

ABSTRACT: Transcriptomic analysis of the effect of cytoplasmic Rat1 (cRat1)

PROVIDER: PRJNA814269 | ENA |

REPOSITORIES: ENA

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Transcriptomic analysis of the effect of cytoplasmic Rat1 (cRat1)

Project description:The main decay pathway of yeast mRNAs in the cytoplasm uses the 5’-3’ exonuclease Xrn1. This protein shuttles from the cytoplasm to the nucleus, where it has a role as transcription factor. We have recently demonstrated that importing depends on two nuclear localization sequences (NLS 1 & NLS2) and that exporting depends on its binding (presumably co-transcriptional) to mRNAs. It is also known that Xrn1 is able to degrade decapped mRNAs that are still being translated by ribosomes. In this work, we analyze the pleiotropic functions of Xrn1 by comparing the phenotypes of yeast strains lacking Xrn1 or its capacity to be imported into the nucleus with those of the substitution of Xrn1 by a cytoplasmic version of the paralogous 5’-3’ exonuclease Rat1 (cRat1). We find that most of the global phenotypes of an xrn1 mutant are partially complemented by cRat1 indicating that this 5’-3’ exonuclease has a similar enzymatic capacity as Xrn1 and that the lack of a cytoplasmic 5’-3’-exoribonuclease is the cause of the physiological defects of an xrn1 mutant. The capacity of cRat1 to perform co-translational decay is, however, very limited. The comparison with the strain carrying Xrn1 with non-functional NLSs (Xrn1ΔNLS1/2) shows that it is slightly deficient in 5’→3’-co-translational decay but much more efficient than cRat1. In both strains, cRat1 and Xrn1ΔNLS1/2, the lack of nuclear Xrn1 has a very minor influence on cell growth experiment in wild type and xrn1 mutant strains.

2024-06-01 | GSE198240 | GEO

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transcriptomic analysis of phosphatin effect-Phosphatin effect on Arabidopsis

Project description:cea11-02_phosphatin - transcriptomic analysis of phosphatin effect - Identification of transcriptomic modifications promoted by phosphatin (AC6) (a molecule mimicking Pi addition effects on Pi starved plants). - 40 ÂµM Phosphatin was added to MS (diluted 10 times) containing 20ÂµM phosphate medium and controls were grown on MS (diluted 10 times) containing 20ÂµM phosphate or supplemented with 500 ÂµM of Pi. For each ARN extraction their was 3 plates containing each 10 seedlings grown 13 days in vertical petri dishes. 6 dye-swap - treated vs untreated comparison

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Transcriptomic analysis revealed adverse effect of melatonin on root growth

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Transcriptomic analysis of the effect of trastuzumab in human iPSC-CMs

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RNA-Seq profiling of single bovine oocyte transcript abundance and its modulation by cytoplasmic polyadenylation

Project description:Polyadenylated RNA from individual germinal vesicle and metaphase II ooyctes was amplified and processed for Illumina sequencing. Increases in polyadenylated transcript abundance were observed and are likely due to cytoplasmic polyadenylation. Transcriptomic analysis of GV and MII bovine oocytes.

2015-07-08 | E-GEOD-61717 | biostudies-arrayexpress

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