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KAT5 activity regulates quiescent-like states in human gliomas [crispr screen]

ABSTRACT: KAT5 activity regulates quiescent-like states in human gliomas [crispr screen]

PROVIDER: PRJNA815683 | ENA |

REPOSITORIES: ENA

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KAT5 activity regulates quiescent-like states in human gliomas [crispr screen]

Project description:In solid tumors, quiescent/G0 cell populations likely play important roles in maintaining cellular heterogeneity and promoting recurrence after stand of care. However, little is known about the mechanisms of tumor cell G0 ingress and egress. To discover regulators of G0-like states for glioblastoma (GBM), we performed a genome-wide CRISPR-Cas9 screen in patient-derived GBM stem-like cells (GSCs) for genes that when inhibited trap cells in G0-like states. We identify the protein acetyltransferase KAT5 as a key regulator of G0 and cell cycle dynamics in GSCs and GSC-derived tumors. In primary gliomas, KAT5low cells display quiescent properties, while overall KAT5 activity increases as tumors become more aggressive. Further, we find that KAT5 activity suppresses the emergence of non-dividing subpopulations with oligodendrocyte progenitor and radial glial cell characteristics both in vitro and in a GSC tumor model. These results reveal that KAT5 activity regulates transitions between non-dividing, neurodevelopmental, and proliferative states in GBM tumors.

2023-12-01 | GSE198523 | GEO

KAT5 activity regulates quiescent-like states in human gliomas [scRNA-seq]

2023-12-01 | GSE198522 | GEO

KAT5 activity regulates quiescent-like states in human gliomas.

Project description:KAT5 activity regulates quiescent-like states in human gliomas.

| PRJNA815681 | ENA

KAT5 activity regulates quiescent-like states in human gliomas [scRNA-seq]

Project description:KAT5 activity regulates quiescent-like states in human gliomas [scRNA-seq]

| PRJNA815684 | ENA

KAT5 activity regulates quiescent-like states in human gliomas.

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-12-01 | GSE198524 | GEO

CRISPR screen

Project description:CRISPR screen

| OEX00023089 | NODE

Expression data from proximal tubular cells of proximal tubular cell-specific KAT5 knockout mice

Project description:Isolated proximal tubular cells from proximal tubular cell-specific KAT5 knockout mice for RNA extraction and hybridization on Affymetrix microarrays. We sought to obtain the physiological significance of KAT5 in proximal tubular cells.

2021-11-19 | GSE168535 | GEO

RNA sequencing of fibroblasts from patients with pathogenic KAT5 variants

Project description:KAT5 encodes an essential lysine acetyltransferase previously called TIP60 involved in gene expression, DNA repair, chromatin remodeling, apoptosis and cell proliferation; but it remains unclear whether variants in this gene causes a genetic disease. Here, we study three individuals with heterozygous de novo missense variants in KAT5 that affect normally invariant residues, with one at the chromodomain (p.Arg53His) and two at or near the acetyl-CoA binding site (p.Cys369Ser and p.Ser413Ala). All three individuals have cerebral malformations, seizures, global developmental delay or intellectual disability, and severe sleep disturbance. Progressive cerebellar atrophy was also noted. Histone acetylation assays with purified mutant KAT5 demonstrated that the variants decrease or abolish the ability of the resulting NuA4/TIP60 multi-subunit complexes to acetylate the histone H4 tail in chromatin. Transcriptomic analysis in patient-derived fibroblasts showed deregulation of multiple genes controlling development. Moreover, there was also upregulated expression of PER1 (a key gene involved in circadian control), in agreement with sleep anomalies in all the patients. In conclusion, dominant missense KAT5 variants cause histone acetylation deficiency with transcriptional dysregulation of multiples genes, thereby leading to a neurodevelopmental syndrome with sleep disturbance, cerebellar atrophy and facial dysmorphisms suggesting a recognizable syndrome.

2020-07-11 | GSE154199 | GEO

FaDu RT CRISPR Screen

Project description:FaDu RT CRISPR Screen

| PRJNA612971 | ENA

HMGA2 project, CRISPR screen

Project description:HMGA2 project, CRISPR screen

| PRJNA718268 | ENA