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ABSTRACT: Effects of dietary glycerol monobutyrin on cecal microbiota of laying hens by 16s rDNA sequencing

PROVIDER: PRJNA816284 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR18329161_1.fastq.gz	Fastqsanger.gz
	SRR18329161_2.fastq.gz	Fastqsanger.gz
	SRR18329162_1.fastq.gz	Fastqsanger.gz
	SRR18329162_2.fastq.gz	Fastqsanger.gz
	SRR18329163_1.fastq.gz	Fastqsanger.gz

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Project description:Cecal microbiota sequencing of laying hens

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16S rDNA (v3-v4 region) sequencing of the cecal bacteria in control (CTL) and chronic nonbacterial prostatitis (CNP) rats

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microRNA-seq profiling of the jejunum mucosa of two laying hen strains fed different dietary Ca and P levels at the egg production peak of 31 weeks of age

Project description:Calcium (Ca) and phosphorus (P) are essential micronutrients linked to arrays of biological processes and physiological conditions. In laying hens, the optimal Ca/P ratio in feed is inconsistent but necessary for reliable schemes of mineral restriction in poultry diets. This study investigates the effects of dietary treatments varying in the Ca and P levels in two laying hen strains (Lohmann Brown-Classic and Lohmann LSL-Classic) at the peak of egg production (31 weeks of age). Four dietary treatment groups were differed in Ca (recommended vs. 15 % reduction) and mineral P (adequate vs. 20 % reduction) levels; 1) control diet (Con; Ca=34.4g/kg, P=5.3 g/kg and Ca/P ratio=7.45), 2) Low Ca and P diet (LCaP), 3) low Ca diet (LCa), and 4) low P diet (LP). microRNA expression of the jejunum mucosa were profiled by microRNA sequencing in a total of 80 animals (10 hens per experimental diet group for each of the two laying line) at sampling age of 31 weeks. RNA-seq data of matched samples are also available (E-MTAB-9109).

2022-01-25 | E-MTAB-9136 | biostudies-arrayexpress

Project description:Cecal microorganisms of laying hens

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mRNA-seq of the jejunum mucosa in LSL and LB laying hens following the variations of dietary calcium and phosphorus levels

Project description:Calcium (Ca) and phosphorus (P) are essential micronutrients that are linked to a wide set of biological processes. In laying hens there is still uncertainty about the optimal Ca/P ratio in feed and further strategies for the reliable mineral restriction in poultry diets are required. The dataset is based on Lohmann Selected Leghorn (LSL) and Lohmann Brown (LB) laying hens sampled at peak performance. The experimental design comprises four dietary groups differed in Ca (recommended vs. 15 % reduction) and mineral P (adequate vs. 20 % reduction) levels; 1) control diet (Con; Ca=34.4g/kg, P=5.3 g/kg and Ca/P ratio=7.45), 2) Low Ca and P diet (LCaP), 3) low Ca diet (LCa), and low P diet (LP). Jejunal transcriptome profiles were assessed by mRNA sequencing in a total of 80 animals (10 hens per experimental group for each laying line) at sampling age of 31 weeks) to approximate the consequences of variable Ca and P supply.

2021-06-14 | E-MTAB-9109 | biostudies-arrayexpress

Mouse caecum vs faeces - Metaproteogenomics Reveals Taxonomic and Functional Changes between Cecal and Fecal Microbiota in Mouse

Project description:We compared the microbiota of paired mouse caecal contents and faeces by applying a multi-omic approach, including 16S rDNA sequencing, shotgun metagenomics, and shotgun metaproteomics. The aim of the study was to verify whether faecal samples are a reliable proxy for the mouse colonic luminal microbiota, as well as to identify changes in taxonomy and functional activity between caecal and faecal microbial communities, which have to be carefully considered when using stool as sample for mouse gut microbiota investigations.

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Project description:cecal microbial diversity of laying hens

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