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Species of moths (Obtectomera)

ABSTRACT: natural host and non-target species parasitized by Cotesia typhae

PROVIDER: PRJNA816685 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR21401952_1.fastq.gz	Fastqsanger.gz
	SRR21401952_2.fastq.gz	Fastqsanger.gz
	SRR21401953_1.fastq.gz	Fastqsanger.gz
	SRR21401953_2.fastq.gz	Fastqsanger.gz
	SRR21401954_1.fastq.gz	Fastqsanger.gz

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Project description:Background: Mosquito midgut is an important target for the host parasite interaction studies as it plays a major role in parasite growth and maturation and vector susceptibility. Proteomic approaches coupled with bioinformatics analysis have been used to study expression of functional proteins/enzymes of An. culicifacies susceptible and refractory species midgut in order to understand the mechanism of refractoriness that may help in contributing to unravel the host pathogen interactions. Methodology/Principle findings: In the present study proteomics approaches namely in solution and in gel digestion strategies followed by LC/MS/MS analysis were used. Further bioinformatics analysis were carried out to find out the functional annotated proteins, biological process, molecular function and their sub-cellular location using Gene ontology, SMART analysis, CELLO etc. In solution and in gel approach coupled with LC-MS/MS identified a total of 91 proteins in susceptible species and 69 proteins in refractory species. Comparative analysis between susceptible and refractory species of An. culicifacies indicated that mainly proteins involved in proteolysis mechanism, catalytic activity, peptidases activity and immune related proteins were found to be dominating in refractory as compared to susceptible species. Conclusion/Significance: Based on the present data a significant increase in number of proteins in midgut of refractory An. culicifacies species B were found that may conclude that these proteins may be responsible for the inhibiting parasite growth and linked to the melanization of oocysts or parasite lysis mechanisms in natural populations of refractory mosquito. Hence the progress of our studies at protein level suggests that these identified annotated putative proteins/enzymes may help to explore natural vector-parasite systems and reveal valuable insights into the mechanism of refractoriness which in turn further useful for bringing of novel strategies for control of malaria.

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Species of moths (Obtectomera)

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