Dataset Information

Insights into the natural defences of a coral reef fish against gill ectoparasites: integrated metabolome and microbiome approach.

ABSTRACT: Understanding natural defence mechanisms against parasites can be a valuable tool for the development of innovative therapies. In this study, we investigated the interplay between the gill mucus metabolome and microbiome of Chaetodon lunulatus, a butterflyfish known to avoid gill monogeneans whilst living amongst closely related parasitized species. In an attempt to identify metabolites and OTUs potentially involved in parasite defence mechanisms, we studied the metabolome (LC-MS/MS) and microbiome of several sympatric butterflyfish species, including the only non-parasitized species C. lunulatus. After observing significant differences between the metabolome and microbiome of parasitized versus non-parasitized fish (PCoA, ANOSIM), we obtained the discriminant metabolites and OTUs using a supervised analysis. Some of the most important discriminant metabolites were identified as peptides, and three new β-subunit haemoblogin-derived peptides from C. lunulatus (CLHbβ-1, CLHbβ-2 and CLHbβ-3) were purified, characterised and synthesised. We also identified specific bacterial families and OTUs typical from low-oxygen habitats in C. lunulatus gill mucus. By using a correlation network between the two datasets, we found a Fusobacteriaceae strain exclusively present in C. lunulatus highly correlated to the peptides. Finally, we discuss the possible involvement of these peptides and Fusobacteriaceae in monogenean avoidance by this fish species.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Chaetodon Lunulatus

TISSUE(S): Gill

SUBMITTER: Sebastien Voisin

LAB HEAD: Philippe Bulet

PROVIDER: PXD018867 | Pride | 2020-11-25

REPOSITORIES: Pride

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Insights into the Natural Defenses of a Coral Reef Fish Against Gill Ectoparasites: Integrated Metabolome and Microbiome Approach.

Reverter Miriam M Sasal Pierre P Suzuki Marcelino T MT Raviglione Delphine D Inguimbert Nicolas N Pare Alan A Banaigs Bernard B Voisin Sébastien N SN Bulet Philippe P Tapissier-Bontemps Nathalie N

Metabolites 20200530 6

Understanding natural defense mechanisms against parasites can be a valuable tool for the development of innovative therapies. We have previously identified a butterflyfish species (<i>Chaetodon</i> <i>lunulatus</i>) that avoids gill monogenean parasites while living amongst closely related parasitized species. The metabolome and microbiome of several sympatric butterflyfish species from the island of Moorea (French Polynesia) were previously described. In this study, we used the previously gene ...[more]

PMID: 32486312

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			Action	DRS
	Cl3160.msf	Msf
	Cl3160.mzML	Mzml
	Cl3160.mzid.gz	Mzid
	Cl3160.pride.mgf.gz	Mgf
	Cl3160.pride.mztab.gz	Mztab