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EPIC-based DNA methylation analysis of glioblastoma stem cells and H9NSC (HOXA-AS2 depletion or overexpression)

ABSTRACT: EPIC-based DNA methylation analysis of glioblastoma stem cells and H9NSC (HOXA-AS2 depletion or overexpression)

PROVIDER: PRJNA818413 | ENA |

REPOSITORIES: ENA

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EPIC-based DNA methylation analysis of glioblastoma stem cells and H9NSC (HOXA-AS2 depletion or overexpression)

Project description:Glioblastoma multiform account for about half of all gliomas and are the most deadly and aggressive forms. Its therapeutic resistance and tumor relapse rely on a subpopulation of cells, the so-called Glioma-stem Cells (GSCs). Here, we investigated for the role of the long non-coding RNA HOXA-AS2 in GSC biology by conducting descriptive and functional analyses of glioma samples classified according to their isocitrate dehydrogenase (IDH1) gene mutation status, and of glioma stem cells. We found that HOXA-AS2 is overexpressed only in aggressive (IDHwt) glioma and GSC. Sh-RNA-based depletion of HOXA-AS2 affects GSC both at the cellular and molecular levels with a decrease in proliferation and altered expression of several hundreds of their genes. Integrative analysis revealed that these changes is expression are not associated to changes in DNA methylation or chromatin signature at the promoter of most deregulated genes following HOXA-AS2 silencing in GSC, supporting a post-transcriptional regulation. In addition, transcription factor motif enrichment and correlation analyses sustained that HOXA-AS2 affect, directly or indirectly, expression of key transcription factors of GCS biology, including E2F8, E2F1, STAT1 and ATF3 to, in fine, contributes to their pathological status by promoting proliferation and modulating the inflammation pathway of Glioma Stem Cell.

2022-04-15 | GSE199032 | GEO

Transcriptome of glioblastoma stem cells and H9NSC (HOXA-AS2 depletion or overexpression)

2022-04-15 | GSE199030 | GEO

Chromatin states in glioblastoma stem cells (HOXA-AS2 depletion)

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Transcriptome and EPIC-based DNA methylation analysis of glioblastoma stem cells and H9NSC (HOXA-AS2 depletion or overexpression), and chromatin states in glioblastoma stem cells (HOXA-AS2 depletion)

Project description:Transcriptome and EPIC-based DNA methylation analysis of glioblastoma stem cells and H9NSC (HOXA-AS2 depletion or overexpression), and chromatin states in glioblastoma stem cells (HOXA-AS2 depletion)

| PRJNA818370 | ENA

Chromatin states in glioblastoma stem cells (HOXA-AS2 depletion)

Project description:Chromatin states in glioblastoma stem cells (HOXA-AS2 depletion)

| PRJNA818368 | ENA

Transcriptome of glioblastoma stem cells and H9NSC (HOXA-AS2 depletion or overexpression)

Project description:Transcriptome of glioblastoma stem cells and H9NSC (HOXA-AS2 depletion or overexpression)

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Transcriptome and EPIC-based DNA methylation analysis of glioblastoma stem cells and H9NSC (HOXA-AS2 depletion or overexpression), and chromatin states in glioblastoma stem cells (HOXA-AS2 depletion)

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-04-15 | GSE199033 | GEO

Analysis of genes regulated by AS1 and AS2

Project description:AS1 and AS2 encode MYB related protein and AS2-domain containing protein, respectively and may regulate transcription. These genes are involved in the determination of axes of leaves of Arabidopsis thaliana. To know the gene regulation in the leaf development, expression profile among wild-type, as1 and as2 mutants and AS2 overexpression plants were compaired. shoot apices from as1-1, as2-1, AS2 overexpressing, and wild type embryos

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Analysis of genes regulated by AS2 and TOP1

Project description:AS2 encode AS2-domain containing protein and may regulate transcription. AS2 is involved in the determination of axes of leaves of Arabidopsis thaliana. Type IB DNA topoisomerase (TOP1α) gene has genetic interaction with AS1 and AS2 and is involved in repression of leaf polarity genes. To know the gene regulation in the leaf development, expression profile among wild-type and as2 mutant treated with and without 10-hydroxyl substituted camptothecin (10H-CPT), a specific TOP1 inhibitor, were compared

2022-12-21 | GSE125138 | GEO

Analysis of genes regulated by AS1 and AS2

2013-03-01 | GSE44028 | GEO