Project description:We performed bulk RNA-sequencing analysis of human submandibular gland samples and then compared to expression of multiple human tissues in order to identify a salivary gland gene signature.
Project description:To compare the gene expression profile of submandibular gland stem cells to submandibular gland epithelia, we have employed whole genome microarray expression profiling as a discovery platform to identify genes with the differential expression in the stem cells and the non-stem cell epithelia. Murine submandibular gland stem cells and non-stem cell epithelia are sorted through FACS
Project description:To compare the gene expression profile of submandibular gland stem cells to submandibular gland epithelia, we have employed whole genome microarray expression profiling as a discovery platform to identify genes with the differential expression in the stem cells and the non-stem cell epithelia.
Project description:In order to explore the functions of carbonic anhydrase VI (CAVI) more fully, we examined the transcriptomic responses to CAVI deficiency in the submandibular gland, stomach, and duodenum of Car6-/- mice by cDNA microarray. 94, 56, and 127 genes were up- or down-regulated in the above-mentioned tissues of Car6-/- mice, respectively. The functional clustering of differentially expressed genes revealed a number of altered biological processes. In the duodenum, the significantly affected biological pathways included immune system process and retinol metabolic process. Response to oxidative stress and brown fat cell differentiation changed remarkably in the submandibular gland. Notably, the submandibular gland, stomach, and duodenum shared one prominent transcriptional susceptibility pathway-catabolic process. Submandibular gland, stomach, and duodenum samples were collected from three wild-type and three Car6-/- female mice, respectively, at the age of two months. Total RNAs were purified and used for cDNA microarray.
Project description:To reveal novel molecular factors behind the development of salivary gland cancer, we performed gene expression analyses from Smgb-Tag mouse salivary gland samples. The overall purpose was to apply these results for clinical use to find new approaches for both possible therapeutic targets and more accurate diagnostic tools in identification of salivary gland cancers. Smgb-Tag mouse strain, in which salivary neoplasms arise through a dysplastic phase in submandibular glands, was investigated using genome-wide microarray expression analysis, Ingenuity pathway analysis, RT-PCR, and immunohistochemistry. 3 normal, 3 dysplastic, and 3 adenocarcinomatous submandibular gland tumours of Smgb-Tag mice.