Project description:Two isogenic human colorectal cancer cell lines (primary SW480 cell line and its lymph node metastatic variant SW620 cell line),as an in vitro metastatic model. We have demonstrated that SW620 cell line possesses high metastasis potential and SW480 cell linepossesses low metastatic potential. We want to compare the whole cell microRNAs profiles of two isogenic colorectal cancer cell lines (SW480 and SW620 cell line), to gain an insight into the molecular events of colon cancer metastasis.
Project description:CDH17 is a key protein in the development of hepatic metastasis in colorectal cancer. In this study, stable silencing of CDH17 was achieved in the KM12SM and SW620 cell lines using shRNA technology, with the objective of exploring the cellular functions and pathways in which CDH17 is implicated.
Project description:Two isogenic human colorectal cancer cell lines (primary SW480 cell line and its lymph node metastatic variant SW620 cell line),as an in vitro metastatic model. We have demonstrated that SW620 cell line possesses high metastasis potential and SW480 cell linepossesses low metastatic potential.
Project description:This study examines the transcriptomic effects of ADAM10 inhibition in two human colorectal cancer cell lines, DLD-1 and SW620. Cells were treated with vehicle control or the conformation-specific ADAM10 monoclonal antibody 1H5 for 48 hours, followed by extraction of total RNA and paired-end RNA sequencing. The dataset enables comparative analysis of ADAM10-regulated pathways across different CRC genetic backgrounds, including alterations in Notch and EGFR signaling, metabolic gene programs, and other pathways associated with tumor progression.
Project description:H3K27 acetylation statuses were analyzed in four colon cancer cell lines (RKO, Caco2, SW48, and SW620), and colorectal cancer-specific super-enhancers were identified.
Project description:DNA methyltransferase 3A (DNMT3A) contributes to tumor immune evasion by regulating TNF signaling and cholesterol biosynthesis in colorectal cancer cells. To characterize DNMT3A-dependent changes in the DNA methylation landscape, genome-wide DNA methylation profiling was performed in control and DNMT3A-knockdown SW620 colorectal cancer cells using the Infinium MethylationEPIC BeadChip array. Comparative analysis identified 14,390 differentially methylated regions, of which 12,059 were annotated to known genes. These methylation alterations were predominantly localized to promoter-associated regions, including TSS1500, TSS200, and 5′ untranslated regions. DNMT3A loss reduced promoter methylation at genes involved in TNFα- and IFNγ-related signaling pathways, including TNFRSF1A and TNFRSF1B. These data define the DNMT3A-dependent DNA methylation landscape in SW620 cells and support a role for DNMT3A-mediated promoter methylation in the transcriptional regulation of tumor immune signaling pathways.
Project description:To reveal the difference in mRNA expression profile between non-senescent cancer cells and senescent cancer cells in colorectal cancer, CRC cell line SW620 was treated with hydrogen peroxide to induce senescent.