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H3K4me3 associated chromatin by CUT&RUN on c-kit+ cells from chimeras

ABSTRACT: H3K4me3 associated chromatin by CUT&RUN on c-kit+ cells from chimeras

PROVIDER: PRJNA819421 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR18468774_1.fastq.gz	Fastqsanger.gz
	SRR18468774_2.fastq.gz	Fastqsanger.gz
	SRR18468775_1.fastq.gz	Fastqsanger.gz
	SRR18468775_2.fastq.gz	Fastqsanger.gz
	SRR18468776_1.fastq.gz	Fastqsanger.gz

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H3K4me3 associated chromatin by CUT&RUN on c-kit+ cells from chimeras

Project description:Sequencing of chromatin associated with H3K4me3 histone marks in live c-kit+ cells isolated by CUT&RUN from chimeras after transplantation with Ctrl-BM, MSC-BM and CpG-BM.

2023-03-24 | GSE199338 | GEO

CUT&RUN-seq of H3K4me3, H3K27me3 and H3K27ac in mll2wt and mll2[Y2602A] mESC

Project description:CUT&RUN-seq of H3K4me3, H3K27me3 and H3K27ac in mouse ESCs carrying a homozygous point mutation in the catalytic domain of mll2[Y2602A]. CUT&RUN was performed according to Skene & Henikoff, 2017 and purified DNA was using for library preparation with NEBNext DNA Ultra II kit (NEB E7645S; input: 5 ng of DNA). Libraries were multiplexed and sequenced on a NextSeq500 (Paired-End; read length 40). Each sample is present in 3 biological replicates.

2023-11-15 | E-MTAB-13466 | biostudies-arrayexpress

MBD2 and H3K4Me3 CUT&RUN-sequencing in C2C12 cells undergoing myogenic differentiation

Project description:MBD2 genome-binding landscape was assessed by CUT&RUN-sequencing in differentiating C2C12 cells. H3K4Me3 CUT&RUN-sequencing was performed as a positive control. Negative control experiment was also performed using a rabbit isotype control monoclonal IgG.

2021-09-08 | GSE183520 | GEO

Profiling of H3K4me3 positioning in primary erythroid cells by CUT&RUN

Project description:The chromatin landscape of developing erythroblasts changes dramatically during differentiation. We have profiled the localisation of H3K4me3 in primary erythroid cells entering terminal differentiation using CUT&RUN.

2022-10-31 | GSE171388 | GEO

CUT&RUN LoV-U of H3K4me3, b-catenin, and IgG in HCT116 cells

Project description:We performed high numbers of replicates of CUT&RUN LoV-U against H3K4me3, β-catenin, and the negative control IgG in human colorectal cancer HCT116 cells over two independent rounds of experiments to discover the complete set of binding events.

2023-07-21 | E-MTAB-13143 | biostudies-arrayexpress

CUT&RUN of TAF4b and H3K4me3 in E16.5 oocytes

Project description:CUT&RUN of TAF4b and H3K4me3 in E16.5 oocytes

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CUT&RUN of TAF4b and H3K4me3 in E16.5 prospermatogonia

Project description:CUT&RUN of TAF4b and H3K4me3 in E16.5 prospermatogonia

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Identification of chromatin states during zebrafish gastrulation using CUT&RUN and CUT&Tag

Project description:Here we describe successful implementation of CUT&RUN for profiling protein-DNA interactions in zebrafish embryos. We apply modified a CUT&RUN method to generate high resolution maps of enrichment for H3K4me3, H3K27me3, H3K9me3, and RNA polymerase II during zebrafish gastrulation. Using this data, we identify a conserved subset of developmental genes that are enriched in both H3K4me3 and H3K27me3 during gastrulation, and we demonstrate the increased effectiveness of CUT&RUN for detecting protein enrichment at repetitive sequences with reduced mappability. Our work demonstrates the power of combining CUT&RUN with the strengths of the zebrafish system to better understand the changing embryonic chromatin landscape and its roles in shaping development.

2021-06-17 | GSE178343 | GEO

CUT&RUN chromatin profiling of H3K4me3, H3K27ac and H3K9me3 histone marks in spermatids and spermatocytes

Project description:Spermatogenesis is a recurring differentiation process that results in the production of male gametes within the testes. During this process, spermatogonial stem cells differentiate to form spermatocytes, which undergo two rounds of meiotic division to form haploid spermatids. Throughout spermiogenesis, round spermatids elongate to form mature sperm. To profile changes in chromatin marks between spermatocytes and spermatids, we generated CUT&RUN data of H3K4me3, H3K27ac and H3K9me3 marks in sorted spermatocytes and spermatids.

2019-01-25 | E-MTAB-6932 | biostudies-arrayexpress

BRD2 CUT & RUN in Calu-3 cells

Project description:we performed CUT&RUN to comprehensively map genomic loci bound to BRD2 in control and BRD2 knockdown Calu-3 cells. We also included H2A.Z, H3K4me3 and IgG antibodies.

2021-09-02 | GSE182993 | GEO

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