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Analysis of total RNA transcriptome in TRIM40 overexpressing HT-29 cells [RNA-seq]

ABSTRACT: Analysis of total RNA transcriptome in TRIM40 overexpressing HT-29 cells [RNA-seq]

PROVIDER: PRJNA822641 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR18583400_1.fastq.gz	Fastqsanger.gz
	SRR18583400_2.fastq.gz	Fastqsanger.gz
	SRR18583401_1.fastq.gz	Fastqsanger.gz
	SRR18583401_2.fastq.gz	Fastqsanger.gz
	SRR18583402_1.fastq.gz	Fastqsanger.gz

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Analysis of total RNA transcriptome in TRIM40 overexpressing HT-29 cells [RNA-seq]

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Project description:Purpose: Identify genes regulated by GPR126 in colon cancer cells by RNA-seq analysis Methods: Use shRNAs to knock down GPR126 in HT-29 cells, total RNAs from scramble group (NC) and GPR126 knockdown group (Sh1) were subjected to RNA-sequencing. Results: Around 700 transcriptomes were up-regulated in GPR126 knockdown HT-29 cells, and 14000 transcriptomes were down-regulated in GPR126 knockdown HT-29 cells.GPR126 mainly regualtes genes from DNA synthesis and cell cycle-related pathways. Conclusions: Our study firstly showed the function of GPR126 regulating colon cancer cell proliferation by targeting genes invovled in DNA synthesis and cell cycle-related pathways.

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