Similar Datasets
Project description:Seven different Solanaceae species, Potato (Solanum tubersosum), Tomato (Lycopersicum esculentum), Eggplant (Solanum melangena), Pepper (Capsicum annuum), Tobacco (Nicotiana tabacum), Petunia and Nicotiana benthamiana were subjected to cold stress. Plants were grown at 25 C for 4-6 weeks after wich cold stress was initiated by exposing the plants to 4 C for 4, 8, 12, 24 and 48 hours. Control samples were isolated from plants just before the cold stress was initated. RNA was isolated using Qiagen RNeasy. Keywords: Direct comparison
2007-09-01 | GSE8203 | GEO
Project description:Lysine acetylation in proteins is a dynamic and reversible Post-translational modification and plays an important role in diverse cellular processes, but limited knowledge is available for acetylation modifications in pepper (Capsicum annuum L.) resistance of cold stress. In this study, the proteome and acetylome of two peppers with different cold resistance under different cold stress and recovery treatment were investigated. In total, 6213 proteins groups and 4574 lysine acetylation sites of 2261 protein groups were identified. Cold stress and recovery treatment results in 3008 differentially expressed proteins (DEPs) and 768 differentially expressed acetylated proteins (DEAPs). Further analysis found a total 1988 proteins were both identifed in the proteome and acetylome data and elucidated the functional differences between the up-regulated and down-regulated proteins in these co-identified proteins through GO enrichment. Twenty acetylation motifs were defined on 3934 unique lysine acetylation sites and motifs *KS*, *KY*, and *KH* occupied the highest proportion of all the identified peptides. Subcellular distribution predictions showed that acetylated proteins in pepper leaves distributed predominantly in chloroplast, cytoplasm and nuclear. KEGG analysis showed 397 identified acetylated proteins were involved in 93 different metabolic pathways. Then the dynamic changes of acetylated proteins in photosynthesis and carbon fixation in photosynthetic organisms pathways under cold stress were further analyzed and many key acetylated proteins regulating cold resistance of pepper were found in these two metabolic pathways. This study is the first to identify the acetylome in pepper, expands greatly the catalog of lysine acetylation substrates and sites in Solanaceae crops and provide a new insight for the post-translational modification study.
2022-02-15 | PXD021024 | Pride
Project description:In this study, we used the illumina high throughput sequencing approach (Sequencing-By-Synthesis, or SBS) to develop the sequence resource of black pepper. To identify micro RNAs functioning in stress response of the black pepper plant, small RNA libraries were prepared from the leaf and root of Phytophthora capsici infected plants, leaves from drought stressed and control plants.
2017-08-24 | GSE65782 | GEO
Project description:PARE (parallel analysis of RNA ends) was performed to study the change of uncapped mRNAs before and after cold treatment in Brachypodium. Different change patterns were identified. We have provided a complete view of uncapped transcriptome under cold stress condition, which will deepen our understanding of gene expression regulation in cold stress response as well as cold stress response mechanism for monocot plants.
2013-07-15 | GSE48234 | GEO
Project description:We report transcripts from tomato:tomato and pepper:pepper self-grafts, and tomato:pepper and pepper:tomato hetergrafts over 4 time points: 24 hours after grafting, 3 days after grafting, 5 day after grafting, and 2 weeks after grafting Examination of 4 graft combinations over 4 time points
2021-08-02 | GSE131700 | GEO
Project description:The male sterility of thermosensitive genic male sterile (TGMS) lines of wheat (Triticum aestivum) is strictly controlled by temperature. The early phase of anther development is especially susceptible to cold stress. MicroRNAs (miRNA) play an important role in plant development and in responses to environmental stress. In this study, deep sequencing of small RNA (smRNA) libraries obtained from spike tissues of the TGMS line under cold and control conditions identified a total of 81 unique miRNA sequences from 30 families, and trans-acting small interfering RNAs (tasiRNAs) derived from two TAS3 genes. We identified 26 targets of 16 miRNA families and three targets of tasiRNAs. Comparing smRNA sequencing datasets and TaqMan qPCR results, we identified six miRNAs and one tasiRNA (tasiRNA-ARF) as cold stress-responsive smRNAs in spike tissues of the TGMS line. We also determined the expression profiles of target genes that encode transcription factors in response to cold stress. Interestingly, expressions of cold-stress responsive smRNAs integrated in the auxin-signaling pathway and their target genes were largely anticorrelated. We investigated tissue-specific expression of smRNAs using a tissue microarray approach. Our data indicated that miR167 and tasiRNA-ARF play roles in regulating the auxin-signaling pathway, and possibly in the developmental response to cold stress. These data provide evidence that smRNA regulatory pathways are linked with male sterility in the TGMS line during cold stress.
2012-03-29 | GSE36867 | GEO
Project description:Upon virus infections, the transcriptomic profile of host plants markedly changes. The rapid and comprehensive transcriptional reprogramming is critical to ward off virus attack. To learn more about transcriptional reprogramming in tobamovirus-infected pepper leaves, we carried out transcriptome-wide RNA-Seq analyses of pepper leaves following Obuda pepper virus (ObPV) and Pepper mild mottle virus (PMMoV)-inoculations.
2021-07-23 | E-MTAB-10769 | biostudies-arrayexpress
Project description:iTRAQ-based quantitative proteomic analysis of heat stress-induced mechanisms in pepper seedlings
2021-05-06 | PXD019556 | Pride